Chiral separation of labetalol stereoisomers in human plasma by capillary electrophoresis
Chiral separation of labetalol stereoisomers in human plasma by capillary electrophoresis
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DOI：
10.1016/j.chroma.2003.08.082
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发表时间：
2004-02-20
影响因子：
4.1
通讯作者：
Matuszewski, BK
中科院分区：
文献类型：
--
作者：
Goel, TV;Nikelly, JG;Matuszewski, BK
在拉贝洛尔立体异构体的手性分离研究中，研究了一种新衍生的环糊精[八基-(2,3-二乙酰基-6-硫酸根)-γ-环糊精]作为毛细管区带电泳中的手性选择剂。七（2,3-二乙酰-6-硫酸根）-β-环糊精（HDAS-β-CD）和八（2,3-二乙酰-6-硫酸根）-γ-环糊精（ODAS-γ-CD）被证明可有效分离拉贝洛尔立体异构体。拉贝洛尔四种立体异构体的最佳分离条件是在低摩尔浓度的酸性 pH 缓冲液中使用 10 mM HDAS-β-CD 和 10 mM ODAS-gamma-CD 实现的。数据说明了毛细管长度和环糊精浓度对分离的影响。更长的毛细管长度和高电压使得所有异构体在不到 15 分钟的时间内实现基线分离。优化后的方法适用于 96 孔固相萃取 (SPE) 分析含有拉贝洛尔的人对照血浆。 (C)2003 Elsevier B.V. 保留所有权利。
A newly derivatized cyclodextrin [octakis-(2,3-diacetyl-6-sulfato)-gamma-cyclodextrin] was investigated as a chiral selector in capillary zone electrophoresis in a study of the chiral separation of labetalol stereoisomers. Heptakis(2,3-diacetyl-6-sulfato)-beta-cyclodextrin (HDAS-beta-CD) and octakis(2,3-diacetyl-6-sulfato)-gamma-cyclodextrin (ODAS-gamma-CD) were shown to be effective in separating labetalol stereoisomers. Optimal separating conditions of the four stereoisomers of labetalol were achieved with 10 mM HDAS-beta-CD and 10 mM ODAS-gamma-CD in an acidic pH buffer of low molarity. Data illustrating the effects of capillary length and cyclodextrin concentration on the separation are presented. The longer capillary length and high voltage enabled the baseline separation of all isomers in less than 15 min. The optimized method was applied to the analysis of human control plasma containing labetalol utilizing solid-phase extraction (SPE) in the 96-well format. (C)2003 Elsevier B.V. All rights reserved.