Immunization with heat shock protein 105-pulsed dendritic cells leads to tumor rejection in mice
Immunization with heat shock protein 105-pulsed dendritic cells leads to tumor rejection in mice
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DOI：
10.1016/j.bbrc.2006.02.142
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发表时间：
2006-04-28
影响因子：
3.1
通讯作者：
Nishimura, Y
中科院分区：
文献类型：
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作者：
Yokomine, K;Nakatsura, T;Nishimura, Y
最近，我们报道了热休克蛋白105（HSP 105）DNA疫苗诱导的抗肿瘤免疫。在本研究中，我们建立了一个临床前研究，以探讨树突状细胞（DC）与小鼠HSP 105作为一个完整的蛋白脉冲在体内癌症免疫治疗的有用性。重组HSP 105不能诱导DC成熟，经HSP 105负载的BM-DC免疫的小鼠可明显抑制皮下肿瘤的生长，并伴有大量CD 4+和CD 8 + T细胞浸润肿瘤。在去除实验中，我们证明了CD 4（+）T细胞和CD 8（+）T细胞在抗肿瘤免疫中起着至关重要的作用。HSP 105致敏的DC可诱导产生HSP 105特异性的CD 4（+）T细胞和CD 8（+）T细胞。因此，与DNA疫苗相比，用HSP 105本身脉冲的BM-DC疫苗接种小鼠可以引起更强的肿瘤排斥。(c)2006年爱思唯尔公司All rights reserved.
Recently, we reported that heat shock protein 105 (HSP105) DNA vaccination induced anti-tumor immunity. In this study, we set tip a preclinical study to investigate the usefulness of dendritic cells (DCs) pulsed with mouse HSP105 as a whole protein for cancer immunotherapy in vivo. The recombinant HSP105 did not induce DC maturation, and the mice vaccinated with HSP105-pulsed BM-DCs were markedly prevented from the growth Of subcutaneous tumors, accompanied with a massive infiltration of both CD4(+) T cells and CD8(+) T cells into the tumors. In depletion experiments, we proved that both CD4(+) T cells and CD8(+) T cells play a crucial role in anti-tumor immunity. Both CD4(+) T cells and CD8(+) T cells specific to HSP105 were induced by stimulation with HSP105-pulsed DCs. As a result, vaccination of mice with BM-DCs pulsed with HSP105 itself could elicit a stronger tumor rejection in comparison to DNA vaccination. (c) 2006 Elsevier Inc. All rights reserved.