Development of capacitance based immunosensors on mixed self-assembled monolayers
Development of capacitance based immunosensors on mixed self-assembled monolayers
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DOI：
10.1016/j.snb.2005.08.039
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发表时间：
2006-04-26
影响因子：
8.4
通讯作者：
Prodromidis, MI
中科院分区：
文献类型：
--
作者：
Hays, HCW;Millner, PA;Prodromidis, MI
报道了金上混合的巯基十六烷酸和 1,2-二棕榈酰-单甘油-3-磷酸乙醇胺-N-己酰生物素自组装单层上基于电容的免疫传感器的构建和表征。利用中性抗生物素蛋白和生物素之间形成的强非共价键将抗人血红蛋白山羊 IgG 固定到混合单层上，从而实现 IgG 抗体的固定。通过物理技术对传感器结构和功能性免疫传感器进行分析，包括石英晶体微天平和用于表面形貌测量的原子力显微镜。使用[I-125]标记的组分独立测定传感器组装和固定化抗体的结合亲和力。最后，在铁氰化物介导的系统中通过电化学阻抗谱分析了部分构建和功能齐全的免疫传感器的电化学特性。血红蛋白传感器在较低的检查频率范围内，在 10(-10) 浓度范围内显示阻抗虚部 (Z'') 的线性响应（根据图 8，线性度在 10(-8) 至 10(-6) M 血红蛋白浓度范围内受到限制）。 (c) 2005 Elsevier B.V. 保留所有权利。
The construction and characterisation of a capacitance based immunosensor on mixed mercaptohexadecanoic acid and 1,2-dipalmitoyl-snglycero-3-phosphoethanolamine-N-caproyl biotinyl self-assembled monolayers on gold are reported. Immobilisation of IgG antibodies was achieved using the strong non-covalent bond formed between Neutravidin and biotin to tether anti-human haemoglobin goat IgG to the mixed monolayers. Analysis of sensor construction and of the functional immunosensors was characterized via physical techniques, including quartz crystal microbalance, and atomic force microscopy for surface topographical measurement. Sensor assembly and the binding affinity of immobilised antibodies were independently determined using [I-125]-tagged components. Finally, the electrochemical properties of partially constructed and fully functional immunosensors were analysed by electrochemical impedance spectroscopy in a ferricyanide mediated system. The haemoglobin sensors show a linear response of the imaginary component of impedance (Z'') across a wide range of concentrations, from 10(-10) (according to Fig. 8, linearity is restricted over the concentration range 10(-8) to 10(-6) M haemoglobin), at the lower range of frequencies examined. (c) 2005 Elsevier B.V. All rights reserved.