Implantation of Autologous Bone-Marrow-Derived Cells Reconstructs Functional Urethral Sphincters in Rabbits
Implantation of Autologous Bone-Marrow-Derived Cells Reconstructs Functional Urethral Sphincters in Rabbits
复制标题
DOI：
10.1089/ten.tea.2010.0478
复制
发表时间：
2011-04-01
影响因子：
4.1
通讯作者：
Nishizawa, Osamu
中科院分区：
文献类型：
--
作者：
Imamura, Tetsuya;Ishizuka, Osamu;Nishizawa, Osamu
本研究的目的是确定自体骨髓来源细胞的植入是否有可能治疗因内括约肌缺陷引起的压力性尿失禁。从新西兰白兔股骨中采集的骨髓细胞培养10天。植入前7天，通过喷洒液氮15秒冷冻损伤位于尿道内口的尿道括约肌。冷冻损伤后7天将培养的自体骨髓来源的细胞植入。对于对照，注射无细胞溶液。植入后7天和14天，测定漏点压力并通过免疫组织化学检查尿道括约肌。第7天和第14天时，细胞植入区域含有大量分别表达肌红蛋白和平滑肌肌动蛋白的横纹肌样细胞和平滑肌样细胞。 7 天和 14 天细胞植入区域中肌红蛋白和平滑肌肌动蛋白表达区域的比例均显着高于对照组。 14 天时，这些分化的细胞与相似的细胞形成接触，形成分层的肌肉结构。此时，细胞植入兔的泄漏点压力明显高于对照组。总之，自体骨髓来源的细胞可以重建功能性尿道括约肌。
The purpose of this study was to determine if implantation of autologous bone-marrow-derived cells has the potential to treat stress urinary incontinence caused by intrinsic sphincter deficiency. Bone marrow cells harvested from femurs of New Zealand White rabbits were cultured for 10 days. Seven days before implantation, the urethral sphincters located at the internal urethral orifice were cryo-injured by spraying liquid nitrogen for 15 s. The cultured autologous bone-marrow-derived cells were implanted 7 days after cryo-injury. For controls, cell-free solutions were injected. At 7 and 14 days after implantation, leak point pressures were determined and the urethral sphincters were examined by immunohistochemistry. At 7 and 14 days, the cell-implanted regions contained numerous striated and smooth muscle-like cells expressing myoglobin and smooth muscle actin, respectively. The proportions of myoglobin- and smooth muscle actin-expressing areas in both the 7- and 14-day cell-implanted regions were significantly higher than in controls. By 14 days, these differentiated cells formed contacts with similar cells, creating layered muscle structures. At that time, the leak point pressure of the cell-implanted rabbits was significantly higher than that of the controls. In conclusion, autologous bone-marrow-derived cells can reconstruct functional urethral sphincters.