A command chemical triggers an innate behavior by sequential activation of multiple peptidergic ensembles
A command chemical triggers an innate behavior by sequential activation of multiple peptidergic ensembles
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DOI：
10.1016/j.cub.2006.06.027
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发表时间：
2006-07-25
期刊：
影响因子：
9.2
通讯作者：
Adams, Michael E.
中科院分区：
文献类型：
--
作者：
Kim, Young-Joon;Zitnan, Dusan;Adams, Michael E.
背景：在每一次蜕皮结束时，昆虫通过蜕皮过程来脱落它们的旧角质层，这是一种由蜕皮前、蜕皮和蜕皮后三个步骤组成的先天行为。血源性蜕皮触发激素(ETH)通过直接作用于中枢神经系统来激活行为序列。结果：为了阐明蜕皮序列背后的神经底物，我们在果蝇中鉴定出表达ETH受体(ETHR)的神经元。不同的eTHR神经元表达多种神经肽，包括激肽、FMR家族、羽化激素(EH)、甲壳类心脏活性多肽(CCAP)、肌肉抑制多肽(MIP)和滑囊素。细胞内钙动态的实时成像显示，在ETH作用后，这些集合被顺序激活。具体地说，FMRFamide神经元在蜕皮前被激活；EH、CCAP和CCAP/MIP神经元在蜕皮前和蜕皮期间活跃；CCAP/MIP/滑囊神经元的活动与蜕皮后相一致。靶向消融特定的eTHR集合会产生与它们在行为序列中所扮演的角色一致的行为缺陷。结论：我们的发现为指挥化学如何通过逐步招募中枢肽能集合来协调先天行为提供了新的见解。
Background: At the end of each molt, insects shed their old cuticle by performing the ecdysis sequence, an innate behavior consisting of three steps: pre-ecdysis, ecdysis, and postecdysis. Blood-borne ecdysis-triggering hormone (ETH) activates the behavioral sequence through direct actions on the central nervous system.Results: To elucidate neural substrates underlying the ecdysis sequence, we identified neurons expressing ETH receptors (ETHRs) in Drosophila. Distinct ensembles of ETHR neurons express numerous neuropeptides including kinin, FMRFamides, eclosion hormone (EH), crustacean cardioactive peptide (CCAP), myoinhibitory peptides (MIP), and bursicon. Real-time imaging of intracellular calcium dynamics revealed sequential activation of these ensembles after ETH action. Specifically, FMRFamide neurons are activated during pre-ecdysis; EH, CCAP, and CCAP/MIP neurons are active prior to and during ecdysis; and activity of CCAP/MIP/bursicon neurons coincides with postecdysis. Targeted ablation of specific ETHR ensembles produces behavioral deficits consistent with their proposed roles in the behavioral sequence.Conclusions: Our findings offer novel insights into how a command chemical orchestrates an innate behavior by stepwise recruitment of central peptidergic ensembles.