S13, a rapidly oncogenic replication-defective avian retrovirus.
S13, a rapidly oncogenic replication-defective avian retrovirus.
复制标题
S13，一种快速致癌的复制缺陷型禽逆转录病毒。
DOI：
10.1016/0042-6822(85)90209-0
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发表时间：
1985
期刊：
影响因子：
3.7
通讯作者：
Vogt,PK
中科院分区：
文献类型：
--
作者：
Beug,H;Hayman,MJ;Graf,T;Benedict,SH;Wallbank,AM;Vogt,PK
禽白血病肉瘤病毒S13在组织培养中转化鸡和日本鹌鹑胚胎成纤维细胞和鸡红系细胞。S13诱导的红系细胞转化需要适合于正常红系细胞前体生长的培养条件（H. Beug和M. J. Hayman（1984），Cell，36，963 - 972）。S13转化的红细胞集落含有高百分比的细胞，其在不存在促红细胞生成素的情况下分化。S13在蛋白质和变性功能上有缺陷，但可以编码一套完整的gag蛋白。经S13转化的非生产细胞克隆释放出一种非感染性的病毒颗粒，该病毒颗粒含有gag但不含功能性的环境蛋白。它们还合成分子量为155，000的转化特异性蛋白质。这种蛋白质与病毒包膜糖蛋白的抗体反应，并表现出良好的抗原性。155，000分子量的v-连接蛋白不与抗v-erbA和v-erbB基因产物的抗血清发生免疫交叉反应。
The avian leukemia sarcoma virus S13 transforms chicken and Japanese quail embryo fibroblasts and chicken erythroid cells in tissue culture. S13-induced erythroid transformation requires culture conditions suitable for the growth of normal erythroid precursors (H. Beug and M. J. Hayman (1984),Cell,36, 963–972). S13-transformed erythroid colonies contain a high percentage of cells that differentiate in absence of erythropoietin. S13 is defective inpolandenvfunctions but can code for a complete set ofgagproteins. Nonproducer cell clones transformed by S13 release a noninfectious viral particle containinggagbut no functionalenvorpolproteins. They also synthesize a transformation-specific protein of 155,000 molecular weight. This protein reacts with antibody to viral envelope glycoproteins and appears to representoncas well asenvsequences. The 155,000-molecular weightenv-linked protein does not cross react immunologically with an antiserum against the v-erbA and v-erbB gene products.