Evaluation of fluorescein-conjugated monoclonal antibody test to detect Chlamydia trachomatis endocervical infections in adolescent girls.
Evaluation of fluorescein-conjugated monoclonal antibody test to detect Chlamydia trachomatis endocervical infections in adolescent girls.
复制标题
荧光素偶联单克隆抗体测试检测青春期女孩沙眼衣原体宫颈内感染的评估。
DOI：
10.1016/s0022-3476(86)81068-x
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发表时间：
1986
期刊：
影响因子：
--
通讯作者：
Schachter,J
中科院分区：
文献类型：
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作者：
Shafer,MA;Vaughan,E;Lipkin,ES;Moscicki,BA;Schachter,J
我们评价了荧光素标记的单抗(FA)试验在普通青少年诊所筛查沙眼衣原体宫颈感染中的作用。连续检查363名青春期女孩，年龄13~20岁，平均17.3岁。45例(12%)FA涂片细胞不足。就诊原因包括非下生殖道相关疾病241例(76%)，下生殖道相关疾病77例(24%)，沙眼衣原体46例(14%)，淋球菌培养22例(7%)，阴道毛滴虫13例(5%)。与我们的组织培养技术相比，FA检测的敏感性为61%(28/46)，特异性为97%(264/272)，阳性预测值为78%(28/36)，阴性预测值为94%(264/282)。尽管黑人测试的敏感率(%)是非黑人的两倍(30%)，但不同种族的测试表现没有显著差异。不同种族在测试表现上的这种明显差异实际上可能代表了群体特征的变化，包括曝光率、敏感度和可用于衣原体组织培养的包涵体形成单位的数量，与非黑人相比。在青少年临床中，组织培养法在检测衣原体方面优于FA。我们建议在不易获得衣原体组织培养分离、已知或预测衣原体感染率较高、以及已知或预测衣原体组织培养中包涵体形成单位数较高的情况下使用FA检测。
We evaluated the fluorescein-conjugated monocional antibody (FA) test for screening forChlamydia trachomatisendocervical infection in a general adolescent clinic. Three hundred sixty-three consecutive adolescent girls, ages 13 to 20 years (mean 17.3 years) were examined. Forty-five (12%) FA smears had insufficient cells. Reason for visit included non-lower genital tract-related disorders in 241 (76%) girls, and lower genital tract-related disorders in 77 (24%).C. trachomatiswas isolated by tissue culture in 46 (14%) patients, andNeisseria gonorrhoeaeby culture in 22 (7%), andTrichomonas vaginaliswas identified by wet mount in 13 (5%). Compared with our tissue culture technique, the performance of the FA test was as follows: sensitivity 61% (28 of 46), specificity 97% (264 of 272), positive predictive value 78% (28 of 36), and negative predictive value 94% (264 of 282). There was no significant difference in test performance by race, although the sensitivity rate (64%) of the test in blacks was twice that (30%) in nonblacks. This apparent difference in test performance by race may actually represent variations in group characteristics, including exposure rate, susceptibility, and number of inclusion forming units available for tissue culture ofChlamydiain blacks compared with nonblacks. In our adolescent clinic, the tissue culture is superior to FA in detectingChlamydia. We recommend that the FA test be used where tissue culture isolation forChlamydiais not readily available, where known or predicted chlamydial infection rates are high, and where known or predicted numbers of inclusion forming units ofChlamydiain tissue culture are high.