Application of the ^19F NMR Technique to Observe Binding of the General Anesthetic Halothane to Human Serum Albumin
Application of the ^19F NMR Technique to Observe Binding of the General Anesthetic Halothane to Human Serum Albumin
复制标题
应用^19F NMR技术观察全身麻醉药氟烷与人血清白蛋白的结合
DOI：
10.2116/analsci.20.1475
复制
发表时间：
2004
影响因子：
1.6
通讯作者：
M. Tashiro
中科院分区：
文献类型：
--
作者：
Kazuaki Shikii;S. Sakurai;H. Utsumi;H. Seki;M. Tashiro
采用^19FNMR技术研究了广泛使用的全身麻醉药氟烷与人血清白蛋白（HSA）的结合性质。结果发现，^19F^1HNOE和2D^1H-^19FHOESY实验检测到了氟烷^19F和HSA质子之间的分子间NOE。还通过^1H和^19F NMR测量了氟烷的扩散系数，表明氟烷与HSA之间存在相互作用。目前的结果表明，这些技术是非常适合的，以确定与蛋白质受体结合的含氟配体在药物发现过程中。
^19F NMR techniques were employed to characterize the binding property of the widely used general anesthetic halothane with human serum albumin (HSA). It was found that ^19F^1H NOE and 2D ^1H-^19F HOESY experiments detected intermolecular NOEs between halothane ^19F and HSA protons. Measurements of the diffusion coefficients for halothane were also carried out by ^1H and ^19F NMR, indicating the interaction of halothane with HSA. The present results indicate that these techniques are very suitable to identify a fluorine-containing ligand binding with a protein receptor in the drug-discovery process.