Long-term cryopreservation can prevent rejection of canine tracheal allografts with preservation of graft viability
Long-term cryopreservation can prevent rejection of canine tracheal allografts with preservation of graft viability
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DOI：
10.1016/s0022-5223(96)70366-5
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发表时间：
1996-05-01
影响因子：
6
通讯作者：
Hitomi, S
中科院分区：
文献类型：
--
作者：
Yokomise, H;Inui, K;Hitomi, S
我们以前报道过，在犬气管自体移植模型中，使用含有海藻糖的保存液可以将气管冷冻保存1个月。意识到冷冻会降低许多器官的同种异体性，我们研究了长期冷冻保存后无免疫抑制剂的犬气管同种异体移植的可能性，从供体狗（n = 12）中取出6至10个气管环，浸入防腐剂溶液中，并在-85 ℃的深冷冻器中冷冻保存285 +/-28天（冷冻组），切除受体犬的5个纵膈气管环，将冷冻后的纵膈气管解冻后移植于受体犬的纵膈气管上。对照组（n = 6），移植物在Euro-Collins液中于10 ℃保存16 ~ 17小时，同深低温保存组进行同种异体气管移植，两组吻合口及移植物均用带蒂网膜覆盖，深低温保存组除1只处死作病理检查外，其余动物均存活2个月以上，对照组动物大多数在移植后1个月内因排斥反应导致气管狭窄而死亡。这些结果表明，将移植物长期冷冻保存在含海藻糖的保存液中，无免疫抑制剂的犬同种异体气管移植是完全可能的。这种简单的方法可以解决供体短缺和免疫抑制问题。
We previously reported that cryopreservation of tracheas for 1 month was possible in a canine tracheal autotransplantation model with use of a preservative solution containing trehalose, Realizing that the allogenicity of many organs is decreased by freezing, we examined the possibility of immunosuppressant-free canine tracheal allotransplantation after long-term cryopreservation, Six to 10 rings of the trachea were removed from donor dogs (n = 12), immersed in the preservative solution, and cryopreserved in a deep freezer at -85 degrees C for 285 +/- 28 days (cryopreservation group), Five rings of the mediastinal trachea of recipient dogs were removed, The cryopreserved tracheas were thawed and transplanted to replace the excised mediastinal tracheas, In a control group (n = 6), the graft was preserved in Euro-Collins solution at 10 degrees C for 16 to 17 hours, Allotransplantation of tracheas was done as in the cryopreservation group, The anastomotic site and graft were covered with an omental pedicle in both groups, In the cryopreservation group, every animal, except one that was killed for pathologic examination, survived more than 2 months, All the grafts of this group were viable, and no stenosis or tracheomalacia was observed, In the control group, most of the animals died within 1 month of tracheal stenosis caused by rejection, These findings reveal that immunosuppressant-free canine tracheal allotransplantation was consistently possible after long-term cryopreservation of the grab in a preservative solution containing trehalose, This simple method could solve both donor shortage and immunosuppression problems.