Gut bacteria differentially affect egg production in the anautogenous mosquito Aedes aegypti and facultatively autogenous mosquito Aedes atropalpus (Diptera: Culicidae).
Gut bacteria differentially affect egg production in the anautogenous mosquito Aedes aegypti and facultatively autogenous mosquito Aedes atropalpus (Diptera: Culicidae).
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DOI：
10.1186/s13071-016-1660-9
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发表时间：
2016-06-30
影响因子：
3.2
通讯作者：
Strand MR
中科院分区：
文献类型：
--
作者：
Coon KL;Brown MR;Strand MR
埃及伊蚊（Aedes aegypti）和埃及伊蚊（A.阿托帕普斯是繁殖能力不同的相关蚊子。埃及伊蚊必须吸血才能产卵（自体），而A.黑须蝎总是在不吸血的情况下产生第一窝卵（自体受精）。我们最近对A.埃及伊蚊A.在实验室中以相同的方式饲养。在这里，我们评估了A.埃及伊蚊A. atropalpus对女性健身，包括产卵。知菌A.埃及伊蚊A. atropalpus幼虫被肠道微生物群的特定成员定殖。然后将每个处理的存活率、发育时间、大小和产蛋量与无菌和常规饲养的幼虫进行比较。我们测试的大多数细菌支持A. aegypti，但只有一种β-变形杆菌，一种丛毛单胞菌，支持A.与传统饲养的雌性相当的水平。斑蚊atropalpus女性殖民丛毛单胞菌含有类似的存储的糖原和蛋白质作为常规饲养的女性，而女性殖民Aquitalea没有。细菌载量的微小差异之间检测到gnotobiotic和传统饲养的A。埃及伊蚊A. atropalpus，但这种变化并不与丛毛单胞菌在A.须萎缩肠道微生物群的特定成员对A. atropalpus比A.埃及人。本文的在线版本（doi：10.1186/s13071-016-1660-9）包含补充材料，可供授权用户使用。
Aedes aegypti and A. atropalpus are related mosquitoes that differ reproductively. Aedes aegypti must blood-feed to produce eggs (anautogenous) while A. atropalpus always produces a first clutch of eggs without blood-feeding (facultatively autogenous). We recently characterized the gut microbiota of A. aegypti and A. atropalpus that were reared identically in the laboratory. Here, we assessed the effects of specific members of the gut microbiota in A. aegypti and A. atropalpus on female fitness including egg production. Gnotobiotic A. aegypti and A. atropalpus larvae were colonized by specific members of the gut microbiota. Survival, development time, size and egg production for each treatment was then compared to axenic and conventionally reared larvae. Most species of bacteria we tested supported normal development and egg production by A. aegypti but only one betaproteobacterium, a Comamonas, supported development and egg production by A. atropalpus to equivalent levels as conventionally reared females. Aedes atropalpus females colonized by Comamonas contained similar stores of glycogen and protein as conventionally reared females, whereas females colonized by Aquitalea did not. Small differences in bacterial loads were detected between gnotobiotic and conventionally reared A. aegypti and A. atropalpus, but this variation did not correlate with the beneficial effects of Comamonas in A. atropalpus. Specific members of the gut microbiota more strongly affected survival, size and egg production by A. atropalpus than A. aegypti. The online version of this article (doi:10.1186/s13071-016-1660-9) contains supplementary material, which is available to authorized users.