Hypoxia-inducible factor-1α-dependent autophagy plays a role in glycolysis switch in mouse granulosa cells
Hypoxia-inducible factor-1α-dependent autophagy plays a role in glycolysis switch in mouse granulosa cells
复制标题
缺氧诱导因子 1α 依赖性自噬在小鼠颗粒细胞糖酵解转换中发挥作用
DOI：
10.1093/biolre/ioy061
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发表时间：
2018-08-01
影响因子：
3.6
通讯作者：
Miao, Yi-Liang
中科院分区：
文献类型：
--
作者：
Zhou, Jilong;Li, Chengyu;Miao, Yi-Liang
Autophagy is an essential cellularmechanism that degrades cytoplasmic proteins and organelles to recycle their components. Here we showed that autophagy was essential for the glycolysis switch and energy homeostasis in mouse granulosa cells under hypoxic condition. Our data indicated that hypoxia inducible factor-1 alpha (HIF-1 alpha) could be largely increased in developing follicles and this remarkable upregulation of HIF-1 alpha triggered cell autophagy and glucose uptake. We found that blocking autophagy by Atg7 knockdown and 3-methyladenine (3-MA) treatment affected the glucose metabolism, with increased glycolytic enzyme activity and decreased ATP production. We also found enhanced lactate level, which was harmful to granulosa cells and could induce cell apoptosis. Thus, our findings highlight a protective role of HIF-1 alpha-dependent autophagy for the granulosa cell glycolysis switch in both energy supply and cell survival.Summary SentenceHIF-1 alpha-induced autophagy is essential for glycolysis metabolism in mouse granulosa cells.
