Mining the salivary proteome with grating-coupled surface plasmon resonance imaging and surface plasmon coupled emission microarrays.
Mining the salivary proteome with grating-coupled surface plasmon resonance imaging and surface plasmon coupled emission microarrays.
复制标题
DOI：
10.1002/0471140856.tx1816s53
复制
发表时间：
2012-08
期刊：
影响因子：
--
通讯作者：
Lynes, Michael A
中科院分区：
文献类型：
--
作者：
Molony, Ryan D;Rice, James M;Yuk, Jong Seol;Shetty, Vivek;Dey, Dipak;Lawrence, David A;Lynes, Michael A
生物学指标在个性化医学中具有众多且广泛的效用，但是这些指标的测量也构成了许多技术和实践挑战。血液/血浆通常被用作测量这些指标的样品来源，但是与样品采购相关的侵入性已导致对唾液的兴趣增加，作为一种有吸引力的替代品。但是，与测量唾液生物标志物有关的独特问题。这些问题与生物标志物谱之间的唾液与血浆之间的不完善相关性更加复杂。在本手稿中，我们解决了与唾液生物标志物量化相关的技术挑战，描述了一种高含量的微阵列测定法，该测定法在高度敏感的测定中采用了光栅耦合的表面等离子体谐振表面等离子体偶联的表面等离子体耦合的发射模态。这种强大的方法提供了绘制唾液蛋白质组的工具，而唾液的蛋白质组应大大增强唾液生物标志物在个性化医学中的效用。
Biological indicators have numerous and widespread utility in personalized medicine, but the measurement of these indicators also pose many technological and practical challenges. Blood/plasma has typically been used as the sample source with which to measure these indicators, but the invasiveness associated with procurement of samples has led to increased interest in saliva as an attractive alternative. However, there are unique issues associated with the measurement of saliva biomarkers. These issues are compounded by the imperfect correlation between saliva and plasma with respect to biomarker profiles. In this manuscript, we address the technical challenges associated with saliva biomarker quantification describe a high-content microarray assay that employs both grating-coupled surface plasmon resonance imaging surface plasmon coupled emission modalities in a highly sensitive assay that has a large dynamic range. This powerful approach provides the tools to map the proteome of saliva, which in turn should greatly enhance the utility of salivary biomarker profiles in personalized medicine.