Creation of an acellular dermal matrix from frozen skin
Creation of an acellular dermal matrix from frozen skin
复制标题
DOI：
10.1007/s002669900292
复制
发表时间：
1999-09-01
影响因子：
2.4
通讯作者：
Uchinuma, E
中科院分区：
文献类型：
--
作者：
Mizuno, H;Takeda, A;Uchinuma, E
目前，大面积皮肤缺损的愈合方法之一是自体表皮移植术。在这项研究中，我们研究了一种处理冷冻多余皮肤的简单方法，以产生无细胞、结构完整的真皮基质。首先，用透射电镜和扫描电镜观察处理后的正常人皮肤脱细胞真皮基质。尽管使用了冷冻皮肤，但该基质保持了真皮层的基底膜复合体和细胞外基质结构。接下来，在动物模型上，我们将ADM和Pelnac移植到裸鼠身上，作为全层伤口的对比剂。真皮基质支持成纤维细胞浸润和新生血管形成。这些结果表明，我们的简单方法处理的皮肤有可能与培养的表皮一起作为真皮模板用于全层伤口的愈合。
At present, one of the treatments of choice for closure of full-thickness skin loss is to use a cultured epidermal autograft when skin loss is extensive. In this study, we investigated a simple method of processing frozen surplus skin to produce an acellular, structurally intact, dermal matrix. First, the acellular dermal matrix prepared from normal human skin (ADM) we processed was observed using a transmission electron microscope and a scanning electron microscope. The matrix maintained the basement membrane complex and the extracellular matrix structure of the dermis despite frozen skin being used. Next, using an animal model, we transplanted the ADM and Pelnac, which is used as a contrast in full-thickness wounds onto nude rats. The dermal matric supported fibroblast infiltration and neovascularization. These results suggest that skin processed by our simple method has the potential to be used as a dermal template together with the cultured epidermis in the closure of full-thickness wounds.