Deinococcus as new chassis for industrial biotechnology: biology, physiology and tools.
Deinococcus as new chassis for industrial biotechnology: biology, physiology and tools.
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DOI：
10.1111/jam.12808
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发表时间：
2015-07
影响因子：
4
通讯作者：
Leonetti JP
中科院分区：
文献类型：
--
作者：
Gerber E;Bernard R;Castang S;Chabot N;Coze F;Dreux-Zigha A;Hauser E;Hivin P;Joseph P;Lazarelli C;Letellier G;Olive J;Leonetti JP
异常球菌属是已发现的最耐辐射的微生物之一。它们对电离辐射、干燥、紫外线辐射和氧化剂造成的一系列损害具有显著的抵抗力。传统上，大肠杆菌和酿酒酵母一直是生物技术应用工程微生物的两个首选平台，因为它们很容易理解和使用。然而，近年来，研究人员已经开始在生物技术和生物修复中使用异常球菌，因为它们具有生长和表达新工程功能的特定能力。最近，几个异常球菌属的测序和比较基因组分析提供了新的见解，这种属的潜力。诸如编码细胞清洁系统的基因的积累等特征在异常球菌属中广泛存在，所述细胞清洁系统消除有机和无机细胞毒性组分。其他特征，如降解和代谢糖和聚合糖的能力，使异常球菌属。这是一种用于工业生物技术的有吸引力的替代品。
Deinococcus spp are among the most radiation-resistant micro-organisms that have been discovered. They show remarkable resistance to a range of damage caused by ionizing radiation, desiccation, UV radiation and oxidizing agents. Traditionally, Escherichia coli and Saccharomyces cerevisiae have been the two platforms of choice for engineering micro-organisms for biotechnological applications, because they are well understood and easy to work with. However, in recent years, researchers have begun using Deinococcus spp in biotechnologies and bioremediation due to their specific ability to grow and express novel engineered functions. More recently, the sequencing of several Deinococcus spp and comparative genomic analysis have provided new insight into the potential of this genus. Features such as the accumulation of genes encoding cell cleaning systems that eliminate organic and inorganic cell toxic components are widespread among Deinococcus spp. Other features such as the ability to degrade and metabolize sugars and polymeric sugars make Deinococcus spp. an attractive alternative for use in industrial biotechnology.