Late Recruitment of Synapsin to Nascent Synapses Is Regulated by Cdk5
Late Recruitment of Synapsin to Nascent Synapses Is Regulated by Cdk5
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DOI：
10.1016/j.celrep.2013.03.031
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发表时间：
2013-04-01
期刊：
影响因子：
8.8
通讯作者：
Washbourne, Philip
中科院分区：
文献类型：
--
作者：
Easley-Neal, Courtney;Fierro, Javier, Jr.;Washbourne, Philip
突触形成是一个复杂的过程，涉及大量突触前和突触后蛋白质的招募和组装。尽管存在于脊椎动物中枢神经系统的每个突触中，但人们对突触蛋白在发育过程中在新生突触中的运输、招募和稳定知之甚少。我们在发育中的斑马鱼脊髓体内检查了突触蛋白向新生突触前末梢的运输和募集。突触蛋白在独立于其他两种突触前细胞器的运输包中运输：突触小泡 (SV) 蛋白质运输囊泡 (STV) 和包含 Piccolo 的活性区前体运输囊泡 (PTV)。在突触前组装期间，所有三个运输包的募集均按有序顺序进行：STV 先于 PTV，而 PTV 又先于突触蛋白。重要的是，细胞周期蛋白依赖性激酶 5 (Cdk5) 特异性调节突触处突触蛋白运输包的后期募集。这些结果表明已建立的突触发生机制具有额外的复杂性。
Synapse formation is a complex process that involves the recruitment and assembly of a myriad of pre- and postsynaptic proteins. Despite being present at every synapse in the vertebrate CNS, little is known about the transport, recruitment, and stabilization of synapsin at nascent synapses during development. We examined the transport and recruitment of synapsin to nascent presynaptic terminals in vivo in the developing zebrafish spinal cord. Synapsin was transported in a transport packet independently of two other presynaptic organelles: synaptic vesicle (SV) protein transport vesicles (STVs) and Piccolo-containing active zone precursor transport vesicles (PTVs). During presynaptic assembly, recruitment of all three transport packets occurred in an ordered sequence: STVs preceded PTVs, which in turn preceded synapsin. Importantly, cyclin-dependent kinase 5 (Cdk5) specifically regulated the late recruitment of synapsin transport packets at synapses. These results point to additional layers of complexity in the established mechanisms of synaptogenesis.