Effects of red light on gibberellin 3?-hydroxylase gene expression in dark-grown seedlings of dwarf and tall cultivars of
Effects of red light on gibberellin 3?-hydroxylase gene expression in dark-grown seedlings of dwarf and tall cultivars of
复制标题
红光对矮、高品种深色幼苗赤霉素3α-羟化酶基因表达的影响
DOI：
10.1078/0176-1617-0758
复制
发表时间：
2002
期刊：
影响因子：
--
通讯作者：
H. Kato‐Noguchi
中科院分区：
文献类型：
--
作者：
H. Kato‐Noguchi
本研究以暗培养、6日龄、高秆（cv.阿拉斯加）和侏儒（CV。进展9）豌豆（Pisum sativum L.）幼苗转移到红光照射条件下。红光对‘进步9号’的抑制作用大于‘阿拉斯加’，48 h内‘进步9号’的伸长量为‘阿拉斯加’的23倍。GA 3β-羟化酶基因的转录水平在暗生长的阿拉斯加的芽中略高于暗生长的进步9号。红光降低了两个品种的转录水平，48 h时的水平分别为约50 μ nt和60 μ nt;与阿拉斯加和进步9号的初始水平相比。这些结果表明，在两个品种的转录水平的差异不明显。Alaska和Progress No.9之间GA 3β-羟化酶蛋白的唯一差异是其氨基酸残基中第229位的丙氨酸被苏氨酸取代，这可能影响酶的活性，并且可能至少部分地有助于这些品种的生长习性。此外，还讨论了鉴别这些品种生长习性的可能性。
Summary The changes in growth and gene expression of gibberellin (GA) 3β-hydroxylase were determined in dark-grown, 6-day-old, tall (cv. Alaska) and dwarf (cv. Progress No. 9) pea ( Pisum sativum L.) seedlings after transfer to conditions of red light irradiation. Red light inhibited the shoot elongation of Progress No. 9 much more so than that of Alaska, and the elongation of Progress No. 9 during 48 h was 23 percnt; that of Alaska. The transcript level of the GA 3β-hydroxylase gene was slightly greater in the shoots of dark-grown Alaska than that of dark-grown Progress No. 9. The red light decreased the transcript levels in both cultivars and the levels at 48 h were about 50 percnt; and 60 percnt; compared to the initial levels for Alaska and Progress No. 9, respectively. These results indicate that the difference in the transcript levels in both cultivars is not apparent. The only difference in GA 3β-hydroxylase proteins between Alaska and Progress No. 9 was the replacement of alanine with threonine at position 229 in their amino acid residues, which may affect the activity of the enzyme and may, at least partially, contribute to the growth habits of those cultivars. A possibility of additional function to distinguish the growth habits of those cultivars is also discussed here.