Dibutoxybutane Suppresses Protein Degradation and Promotes Growth in Cultured Chicken Muscle Cells
Dibutoxybutane Suppresses Protein Degradation and Promotes Growth in Cultured Chicken Muscle Cells
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DOI：
10.2141/jpsa.0120063
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发表时间：
2013-01-01
影响因子：
1.5
通讯作者：
Hayashi, Kunioki
中科院分区：
文献类型：
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作者：
Kamizono, Tomomi;Ohtsuka, Akira;Hayashi, Kunioki
二丁氧基丁烷 (DBB) 是烧酒酒厂副产品中可能含有的一种生长促进剂。在本研究中，我们合成了 DBB 以确认其生长促进作用。通过核磁共振和气相色谱质谱确定合成产物的化学结构后，利用鸡骨骼肌细胞进行了实验。将DBB以0、0.015、0.15、1.5、15和150μg/ml的水平与培养基混合，并将源自13日龄鸡胚大腿肌肉的细胞培养6天。 DBB 加速了肌肉细胞的生长，并且降低了从 N-tau-甲基组氨酸释放到培养基中估计的蛋白质降解率。此外，DBB 还减少了肌肉特异性 E3 泛素连接酶、atrogin-1/MAFbx 和 MuRF1 的 mRNA。然而，泛素、蛋白酶体C2亚基和mu-钙蛋白酶的mRNA不受DBB的影响。 DBB 降低了鸡中两种普遍存在的 mu- 和 mu/m-钙蛋白酶（通过酪蛋白酶谱定量）的活性。这些结果表明，DBB 由于蛋白质降解率降低而促进肌肉生长。
Dibutoxybutane (DBB) is a possible growth promoter contained in shochu distillery by-product. In the present study, we synthesized DBB in order to confirm the growth promoting action. After determining the chemical structure of the synthetic product by nuclear magnetic resonance and gas chromatography mass spectrometry, an experiment was conducted using chicken skeletal muscle cells. DBB was mixed with the culture medium at the level of 0, 0.015, 0.15, 1.5, 15 and 150 mu g/ml and cells derived from thigh muscle of 13-day-old chick embryos were raised for 6 days. The muscle cell growth was accelerated and the rate of protein degradation estimated from N-tau-methylhistidine release into the medium was decreased by DBB. Furthermore, the mRNAs of muscle-specific E3 ubiquitin ligases, atrogin-1/MAFbx and MuRF1, were decreased by DBB. However, mRNAs of ubiquitin, proteasome C2 subunit and mu-calpain were not affected by DBB. Activities of chicken's two ubiquitous mu- and mu/m-calpain which were quantified by casein zymography were decreased by DBB. These results show that DBB promotes muscle growth due to a decrease in the rate of protein degradation.