Enhancing Alkyne-Based Raman Tags with a Sulfur Linker
Enhancing Alkyne-Based Raman Tags with a Sulfur Linker
复制标题
使用硫链接剂增强基于炔烃的拉曼标签
DOI：
10.1021/acs.jpcb.2c09093
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发表时间：
2023
期刊：
影响因子：
--
通讯作者：
Potma, Eric O.
中科院分区：
文献类型：
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作者：
Li, Yong;Townsend, Katherine M.;Dorn, Robert S.;Prescher, Jennifer A.;Potma, Eric O.
基于炔的拉曼标记已经证明了它们用于生物成像的实用性。尽管炔基伸缩模式是相对强的拉曼散射体，但炔标记的化合物的检测灵敏度最终受限于探针的拉曼响应的幅度。为了提高基于炔的拉曼探针的性能，我们设计了几种受益于π-π共轭以及与硫接头的额外n−π共轭的标签。我们发现，硫连接提供了额外的增强和线宽变窄，提供了一个简单而有效的策略，以改善基于炔的拉曼标签。我们通过受激拉曼散射显微镜验证了各种硫连接的炔标记用于细胞成像的效用。
Alkyne-based Raman tags have proven their utility for biological imaging. Although the alkynyl stretching mode is a relatively strong Raman scatterer, the detection sensitivity of alkyne-tagged compounds is ultimately limited by the magnitude of the probe’s Raman response. In order to improve the performance of alkyne-based Raman probes, we have designed several tags that benefit from π–π conjugation as well as from additional n−π conjugation with a sulfur linker. We show that the sulfur linker provides additional enhancement and line width narrowing, offering a simple yet effective strategy for improving alkyne-based Raman tags. We validate the utility of various sulfur-linked alkyne tags for cellular imaging through stimulated Raman scattering microscopy.