A CsMYB6-CsTRY module regulates fruit trichome initiation in cucumber.
A CsMYB6-CsTRY module regulates fruit trichome initiation in cucumber.
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CsMYB6-CsTRY 模块调节黄瓜果实毛状体的起始
DOI：
10.1093/jxb/ery047
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发表时间：
2018-04-09
影响因子：
6.9
通讯作者：
Ren H
中科院分区：
文献类型：
--
作者：
Yang S;Cai Y;Liu X;Dong M;Zhang Y;Chen S;Zhang W;Li Y;Tang M;Zhai X;Weng Y;Ren H
CsMYB 6与CsTRY相互作用，协同抑制黄瓜果实毛状体的发生。黄瓜果实表皮毛或刺的数量和大小是黄瓜生产中重要的果实品质性状。黄瓜果刺形成的分子机制目前还知之甚少。在这里，我们报告的黄瓜CsMYB 6基因，它编码一个混合物样MYB转录因子，在调节水果毛状体发育中起着重要作用的功能特性。时空表达分析显示，高水平表达的CsMYB 6在表皮的黄瓜子房在果刺启动，这是类似的表达CsTRY，拟南芥TRY基因的同源物，也起着关键作用，在毛状体的发展。CsMYB 6和CsTRY在黄瓜和拟南芥中的过表达揭示了CsMYB 6和CsTRY在这两个物种中作为毛状体起始的负调节因子，并且CsMYB 6在此过程中在CsTRY的上游起作用。CsMYB 6与CsTRY启动子区域内的3个MYB结合位点结合，蛋白质相互作用实验表明CsTRY与CsMYB 6蛋白直接相互作用。结果还揭示了CsMYB 6及其拟南芥同源物AtMYB 106在毛状体发育中的保守和不同作用。总的来说，我们的研究结果揭示了一种新的机制，其中CsMYB 6-CsTRY复合物负调控黄瓜果实毛状体的形成。
CsMYB6 interacts with CsTRY and they work cooperatively to suppress fruit trichome initiation in cucumber. Fruit epidermal features such as the number and size of trichomes or spines are important fruit quality traits in cucumber production. Little is known about the molecular mechanisms underlying fruit spine formation in cucumber. Here, we report functional characterization of the cucumber CsMYB6 gene, which encodes a MIXTA-like MYB transcription factor that plays an important role in regulating fruit trichome development. Spatial-temporal expression analyses revealed high-level expression of CsMYB6 in the epidermis of cucumber ovaries during fruit spine initiation, which was similar to the expression of CsTRY, a homolog of the Arabidopsis TRY gene that also plays a key role in trichome development. Overexpression of CsMYB6 and CsTRY in cucumber and Arabidopsis revealed that CsMYB6 and CsTRY act as negative regulators of trichome initiation in both species, and that CsMYB6 acted upstream of CsTRY in this process. CsMYB6 was found to bind to the three MYB binding sites inside the promoter region of CsTRY, and protein–protein interaction assays suggested that CsTRY also directly interacted with CsMYB6 protein. The results also revealed conserved and divergent roles of CsMYB6 and its Arabidopsis homolog AtMYB106 in trichome development. Collectively, our results reveal a novel mechanism in which the CsMYB6-CsTRY complex negatively regulates fruit trichome formation in cucumber.
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影响因子：
3.7
作者：
Li Q;Zhang C;Li J;Wang L;Ren Z
通讯作者：
Ren Z
影响因子：
7.2
作者：
Machado, Adriane;Wu, Yingru;Dennis, Elizabeth S.
通讯作者：
Dennis, Elizabeth S.
影响因子：
64.5
作者：
HULSKAMP, M;MISERA, S;JURGENS, G
通讯作者：
JURGENS, G
影响因子：
1.2
作者：
Bodnaryk, R
通讯作者：
Bodnaryk, R
影响因子：
7.2
作者：
Clough, SJ;Bent, AF
通讯作者：
Bent, AF