Genome damage accumulated in seed ageing leads to plant genome instability and growth inhibition.
Genome damage accumulated in seed ageing leads to plant genome instability and growth inhibition.
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DOI：
10.1042/bcj20230006
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发表时间：
2023-04-12
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成功的发芽和幼苗建立是作物产量和植物在自然环境中存活的重要决定因素。发芽潜力受到次优环境条件的影响，导致种子老化和高水平的基因组损伤。然而，在种子中积累的DNA损伤对随后的幼苗生长的致突变性和生长抑制潜力在很大程度上仍然未知。染色体断裂修复因子DNA LIGASE 4和DNA LIGASE 6缺陷的拟南芥种子表现出对自然老化效应的超敏反应，相对于野生型种子，发芽势和幼苗生物量降低。在这里，我们确定，老年拟南芥种子显示升高水平的程序性细胞死亡（PCD）的根分生组织，持续到幼苗建立，与更高水平的细胞死亡的线缺乏DNA双链断裂修复。报告行确定种子老化的突变水平和染色体内重组频率的影响。种子劣化导致萌发幼苗中移码突变和基因组不稳定性水平显著升高。因此，在植物生命周期的种子阶段发生的基因组损伤水平升高可能对随后的植物发育产生显著影响。此外，种子老化的诱变效应对植物种群的基因组稳定性和生态系统适应性具有潜在的长期影响。总的来说，我们确定了次优质量种子中积累的基因组损伤对随后植物生长和基因组稳定性的影响，以及对作物产量和植物在气候变化下生存的相关影响。
Successful germination and seedling establishment are important determinants of crop yields and plant survival in natural environments. Germination potential is compromised by suboptimal environmental conditions that result in seed ageing and high levels of genome damage. However, the mutagenic and growth inhibitory potential of DNA damage accumulated in seeds on subsequent seedling growth remains largely unknown. Arabidopsis seeds deficient in the chromosomal break repair factors DNA LIGASE 4 and DNA LIGASE 6 exhibited hypersensitivity to the effects of natural ageing, with reduced germination vigour and seedling biomass relative to wild type seed. Here, we identify that aged Arabidopsis seed display elevated levels of programmed cell death (PCD) in the root meristem which persists into seedling establishment, with higher levels of cell death in lines deficient in DNA double strand break repair. Reporter lines determined the effects of seed ageing on mutation levels and intrachromosomal recombination frequencies. Seed deterioration resulted in strikingly elevated levels of frameshift mutations and genome instability in germinated seedlings. Thus, elevated levels genome damage incurred in the seed stage of the plant life cycle potentially impacts significantly on subsequent plant development. Furthermore, the mutagenic effects of seed ageing has potentially long-term implications on the genome stability of plant populations and ecosystem fitness. Collectively, we identify genome damage accumulated in suboptimal quality seed impacts on subsequent plant growth and genome stability, with associated implications for crop yields and plant survival under changing climates.