5-bromo-2'-deoxyuridine blocks myogenesis by extinguishing expression of MyoD1.
5-bromo-2'-deoxyuridine blocks myogenesis by extinguishing expression of MyoD1.
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5-bromo-2-deoxyuridine 通过抑制 MyoD1 的表达来阻断肌生成。
DOI：
10.1126/science.2547249
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发表时间：
1989
期刊：
影响因子：
56.9
通讯作者：
H. Weintraub
中科院分区：
文献类型：
--
作者：
S. Tapscott;A. Lassar;R. Davis;H. Weintraub
嘧啶类似物5-溴脱氧尿苷(BUDR)与胸腺嘧啶核苷竞争掺入DNA。用BUDR替代胸腺嘧啶核苷不会显著影响细胞活力，但会阻碍许多不同谱系的细胞分化。在小鼠成肌细胞系中，BUDR的替换阻止了成肌分化，并消除了成肌决定基因MyoD1的表达。在budR替代的成肌细胞中强制表达MyoD1，克服了budr诱导分化的障碍。在转基因成肌细胞中，观察到BUDR替代的肌肉结构基因的激活和明显的正常分化。这表明，BUDR在生肌调控基因水平上阻止肌肉发生，可能是MyoD1，而不是通过直接抑制肌肉结构基因的激活。这与BUDR选择性地阻断一类调控基因的想法是一致的，这些调控基因的每个成员对不同细胞系的发育都很重要。
The pyrimidine analog 5-bromodeoxyuridine (BUdR) competes with thymidine for incorporation into DNA. Substitution of BUdR for thymidine does not significantly affect cell viability but does block cell differentiation in many different lineages. BUdR substitution in a mouse myoblast line blocked myogenic differentiation and extinguished the expression of the myogenic determination gene MyoD1. Forced expression of MyoD1 from a transfected expression vector in a BUdR-substituted myoblast overcame the block to differentiation imposed by BUdR. Activation of BUdR-substituted muscle structural genes and apparently normal differentiation were observed in transfected myoblasts. This shows that BUdR blocks myogenesis at the level of a myogenic regulatory gene, possibly MyoD1, not by directly inhibiting the activation of muscle structural genes. It is consistent with the idea that BUdR selectively blocks a class of regulatory genes, each member of which is important for the development of a different cell lineage.
真核 DNA 复制起源研究的新起点。
DOI：
10.1016/0167-4781(89)90123-1
发表时间：
1989
期刊：
Biochimica et biophysica acta
影响因子：
--
作者：
Umek,RM;Linskens,MH;Kowalski,D;Huberman,JA
通讯作者：
Huberman,JA