Anterograde tracing of horseradish peroxidase (HRP) with the electron microscope using the tetramethylbenzidine reaction
Anterograde tracing of horseradish peroxidase (HRP) with the electron microscope using the tetramethylbenzidine reaction
复制标题
使用四甲基联苯胺反应用电子显微镜顺行追踪辣根过氧化物酶 (HRP)
DOI：
10.1016/0165-0270(81)90007-8
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发表时间：
1981
影响因子：
3
通讯作者：
H. Holländer
中科院分区：
文献类型：
--
作者：
K. Schönitzer;H. Holländer
用四甲基联苯胺（TMB）组织化学方法，对家兔初级视皮层或单眼注射辣根过氧化物酶（HRP）后，在相邻切片上进行了上级丘的光镜和电镜观察。电镜下观察到标记的轴突和轴突终末，TMB反应产物为晶体状电子致密结构。在对侧丘的对照切片中，神经纤维素没有标记。
A technique is described for anterograde horseradish peroxidase (HRP) tracing with the electron microscope using tetramethylbenzidine (TMB) histochemistry.Following HRP injection into the primary visual cortex or one eye of rabbits the superior colliculus was studied with the light and electron microscope in adjacent sections. In target preparations from the colliculus examined with the electron microscope labelled axons and axon terminals were found. The TMB reaction product was identified as crystal like electron-dense structures. In control sections from the opposite colliculus the neuropil was free from label.