Alterations in the insulin-like growth factor system during the menstrual cycle in normal women
Alterations in the insulin-like growth factor system during the menstrual cycle in normal women
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DOI：
10.1016/s0378-5122(97)00088-1
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发表时间：
1998-01-12
期刊：
影响因子：
4.9
通讯作者：
Lonning, PE
中科院分区：
文献类型：
--
作者：
Helle, SI;Anker, GB;Lonning, PE
目的：评价内源性雌激素和孕激素对未使用避孕药的健康绝经前妇女正常月经周期中IGF系统的影响。方法：在5周的研究期间，9名妇女以2-3天的间隔获得空腹血液样本。采用放射免疫分析法（RIA）测定血浆IGF-I、IGF-II、IGFBP-1、IGFBP-3、雌二醇和孕酮水平。IGFBP-3也通过Western配体印迹（WLB）和免疫印迹进行评估。通过Friedman检验评价月经期（定义为第1-5天）、卵泡期和黄体期（基于血浆雌二醇和孕酮值分离）之间的任何差异。结果如下：用RIA法测得的血浆IGF-I（P < 0.01）和IGFBP-3（P < 0.05）水平在卵泡期最高。未发现IGFBP-1和IGF-II的血浆水平发生变化，免疫印迹未显示月经周期中片段化IGFBP-3与完整IGFBP-3的比例发生任何变化。9例患者中有8例患者的血浆IGF-I水平与雌二醇水平呈正相关（P = 0.012）。结论：与月经期相比，卵泡期和黄体期血浆IGF-I水平略高但显著较高，这一发现表明血浆雌二醇可能影响该生长因子的水平。在月经周期的不同时间，个体患者的血浆IGF-I水平与雌二醇而非孕酮水平之间存在相关性，这一发现进一步支持了这一假设。(C)1998爱思唯尔科学爱尔兰有限公司保留所有权利。
Objectives: To evaluate the effects of endogenous estrogens and progestins on the IGF-system during the normal menstrual cycle in healthy premenopausal women not using contraceptive drugs. Methods: Nine women had fasting blood samples obtained at 2-3 days intervals during a 5 week study period. Plasma levels of IGF-I, IGF-II, IGFBP-1, IGFBP-3, estradiol and progesterone were measured by radioimmunoassay (RIA) in each sample. IGFBP-3 was also evaluated by Western ligand blot (WLB) and immunoblot. Any differences between the menstrual phase (defined as day 1-5), follicular and luteal phases (separation based on plasma estradiol and progesterone values) were evaluated by the Friedman test. Results: A small but significant difference in plasma levels of IGF-I (P < 0.01) and IGFBP-3 (P < 0.05) measured by RIA between the three phases were seen with the highest levels found during the follicular phase. No change in plasma levels of IGFBP-1 and IGF-II was found and immunoblots did not reveal any alteration in the ratio of fragmented to intact IGFBP-3 during the menstrual cycle. A positive correlation between plasma levels of IGF-I and estradiol was seen in 8 out of 9 patients (P = 0.012). Conclusions: The finding of a slight but significant higher level of plasma IGF-I in the follicular and luteal phases compared with the menstrual phase suggests plasma estradiol may influence the level of this growth factor. This hypothesis is further supported by the finding of a correlation between plasma levels of IGF-I and estradiol but not progesterone in individual patients at different times during the menstrual cycle. (C) 1998 Elsevier Science Ireland Ltd. All rights reserved.