The widespread impact of mammalian microRNAs on mRNA repression and evolution
The widespread impact of mammalian microRNAs on mRNA repression and evolution
复制标题
DOI：
10.1126/science.1121158
复制
发表时间：
2005-12-16
期刊：
影响因子：
56.9
通讯作者：
Bartel, DP
中科院分区：
文献类型：
--
作者：
Farh, KKH;Grimson, A;Bartel, DP
成千上万的哺乳动物信使RNA处于选择性压力下，以维持与microRNA（miRNA）相匹配的7个核苷酸位点。我们发现，这些保守的靶标通常在miRNA表达之前在发育阶段高度表达，并且它们的水平倾向于靶向它们的miRNA开始积累。未经保守的地点超过了保守地点10到1，也介导了抑制。结果，与miRNA同时和位置同时和位置的基因已经演变为有选择地避免与miRNA相匹配的位点。这种选择性回避的现象扩展到数千个基因，并通过找到组织和发育阶段来揭示miRNA的空间和时间特异性，在这些阶段中，与相应位点的信息在较低级别表达。
Thousands of mammalian messenger RNAs are under selective pressure to maintain 7-nucleotide sites matching microRNAs (miRNAs). We found that these conserved targets are often highly expressed at developmental stages before miRNA expression and that their levels tend to fall as the miRNA that targets them begins to accumulate. Nonconserved sites, which outnumber the conserved sites 10 to 1, also mediate repression. As a consequence, genes preferentially expressed at the same time and place as a miRNA have evolved to selectively avoid sites matching the miRNA. This phenomenon of selective avoidance extends to thousands of genes and enables spatial and temporal specificities of miRNAs to be revealed by finding tissues and developmental stages in which messages with, corresponding sites are expressed at lower levels.