Advanced imaging and tissue engineering of the human limbal epithelial stem cell niche.
Advanced imaging and tissue engineering of the human limbal epithelial stem cell niche.
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DOI：
10.1007/978-1-4939-1785-3_15
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发表时间：
2015
期刊：
影响因子：
--
通讯作者：
Daniels, Julie T
中科院分区：
文献类型：
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作者：
Massie, Isobel;Dziasko, Marc;Kureshi, Alvena;Levis, Hannah J;Morgan, Louise;Neale, Michael;Sheth, Radhika;Tovell, Victoria E;Vernon, Amanda J;Funderburgh, James L;Daniels, Julie T
角膜缘上皮干细胞龛提供了一个独特的物理保护环境，其中角膜缘上皮干细胞与辅助细胞类型及其分泌因子紧密相邻。先进的成像技术的使用被描述为可视化的生态位在自然人角膜组织的三维。此外，提供了用于分离和培养三种不同细胞类型的方案，包括来自人供体组织的角膜缘龛的人角膜缘上皮干细胞。最后，将这些细胞内的塑料压缩胶原蛋白结构，以形成一个组织工程化的角膜利姆布斯的过程中，以及如何免疫组织化学技术可以应用于表征细胞表型。
The limbal epithelial stem cell niche provides a unique, physically protective environment in which limbal epithelial stem cells reside in close proximity with accessory cell types and their secreted factors. The use of advanced imaging techniques is described to visualize the niche in three dimensions in native human corneal tissue. In addition, a protocol is provided for the isolation and culture of three different cell types, including human limbal epithelial stem cells from the limbal niche of human donor tissue. Finally, the process of incorporating these cells within plastic compressed collagen constructs to form a tissue-engineered corneal limbus is described and how immunohistochemical techniques may be applied to characterize cell phenotype therein.