A cyclic-di-GMP receptor required for bacterial exopolysaccharide production.
A cyclic-di-GMP receptor required for bacterial exopolysaccharide production.
复制标题
细菌外多糖产生所需的环状-DI-GMP受体。
DOI：
10.1111/j.1365-2958.2007.05879.x
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发表时间：
2007-09
影响因子：
3.6
通讯作者：
Lory, Stephen
中科院分区：
文献类型：
--
作者：
Lee, Vincent T;Matewish, Jody M;Kessler, Jennifer L;Hyodo, Mamoru;Hayakawa, Yoshihiro;Lory, Stephen
双-(3‘，5’)-环-二聚鸟苷单磷酸(c-di-GMP)是一种全球性的调节分子，可以调节细菌的相互反应，激活毒力途径或生物膜的形成。C-di-GMP的信号转导机制，包括对c-di-GMP的识别和随后的表型调控，在很大程度上仍不清楚。这些调控通路的关键成分是各种适配蛋白(c-di-GMP受体)。有令人信服的证据表明，除了Pilz结构域，还有其他未知的c-di-GMP受体。我们发现铜绿假单胞菌的PelD蛋白是一种新的c-di-GMP受体，它介导c-di-GMP调节PEL多糖的生物合成。对PelD同源物的分析确定了一些保守残基，这些残基是c-di-GMP结合和合成PEL多糖所必需的。PelD的二级结构与二鸟苷环化酶抑制部位的相似性表明，一个共同的折叠可以作为结合c-di-GMP的平台。C-di-GMP结合位点与一个蛋白结构域中的各种输出信号基序的结合为不同细胞对这种调节二核苷酸的反应提供了一种特异性的解释。
Bis-(3′,5′)-cyclic-dimeric-guanosine monophosphate (c-di-GMP) has been shown to be a global regulatory molecule that modulates the reciprocal responses of bacteria to activate either virulence pathways or biofilm formation. The mechanism of c-di-GMP signal transduction, including recognition of c-di-GMP and subsequent phenotypic regulation, remain largely uncharacterized. The key components of these regulatory pathways are the various adaptor proteins (c-di-GMP receptors). There is compelling evidence suggesting that, in addition to PilZ domains, there are other unidentified c-di-GMP receptors. Here we show that the PelD protein of Pseudomonas aeruginosa is a novel c-di-GMP receptor that mediates c-di-GMP regulation of PEL polysaccharide biosynthesis. Analysis of PelD orthologues identified a number of conserved residues that are required for c-di-GMP binding as well as synthesis of the PEL polysaccharide. Secondary structure similarities of PelD to the inhibitory site of diguanylate cyclase suggest that a common fold can act as a platform to bind c-di-GMP. The combination of a c-di-GMP binding site with a variety of output signalling motifs within one protein domain provides an explanation for the specificity for different cellular responses to this regulatory dinucleotide.