Effects of fasting and refeeding on gene expression of slc15a1a, a gene encoding an oligopeptide transporter (PepT1), in the intestine of Mozambique tilapia
Effects of fasting and refeeding on gene expression of slc15a1a, a gene encoding an oligopeptide transporter (PepT1), in the intestine of Mozambique tilapia
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DOI：
10.1016/j.cbpb.2016.09.006
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发表时间：
2017-01-01
影响因子：
2.2
通讯作者：
Watanabe, Soichi
中科院分区：
文献类型：
--
作者：
Orozco, Zenith Gaye A.;Soma, Satoshi;Watanabe, Soichi
为了更好地了解寡肽转运蛋白PepT 1基因slc 15 a1 a在莫桑比克罗非鱼肠上皮中的组织分布及其对禁食和再投喂的反应，研究了其在营养吸收中的作用。slc 15 a1 a主要表达在肠前部的吸收上皮细胞中，表明消化的寡肽主要在前肠中吸收。分别于末次进食后第1、2、4、7、14天检测slc 15 a1 a对禁食的反应。禁食揭示了双相效应，短期禁食显著上调slc 15 a1 a表达，长期禁食导致下调。表达水平持续降低，从第4天至第14天降至禁食前水平以下。近端（肝环，HL）和远端部分（近端主要线圈，PMC）的前肠表现出不同程度的反应，禁食; slc 15 a1 a在PMC的表达表现出更大的上调和下调比在HL。再喂养显著刺激slc 15 a1 a的表达在第3天，虽然表达没有超过禁食前的水平。观察到的slc 15 a1 a对禁食和再喂养的反应表明，该基因的表达水平可以作为改变营养条件时可能发生的变化的敏感指标。这些发现有助于更好地了解PepT 1在营养中的作用以及肠道中寡肽和氨基酸吸收的复杂机制，并可能导致开发可能的方法来操纵吸收过程，以改善鱼类的生长和其他代谢和生理条件。(C)2016由Elsevier Inc.出版
The tissue distribution of slc15a1 a, a gene that encodes an oligopeptide transporter, PepT1, and its response to fasting and refeeding were investigated in the intestinal epithelium of Mozambique tilapia for a better understanding of its role on nutrient absorption. The slc15a1a was predominantly expressed in the absorptive epithelia of the anterior part of the intestine, suggesting that digested oligopeptides are primarily absorbed in the anterior intestine. The response of slc15a1a to fasting was evaluated at 1, 2,4, 7 and 14 days after the last feeding. Fasting revealed a biphasic effect, where short-term fasting significantly upregulated slc15a1a expression and long-term fasting resulted in downregulation. The expression level continued to decrease and fell below the pre-fasted level from day 4 to 14. Proximal (the hepatic loop, HL) and distal parts (the proximal major coil, PMC) of the anterior intestine showed different magnitudes of responses to fasting; slc15a1a expression in the PMC showed greater upregulation and downregulation than that in the HL. Refeeding significantly stimulated slc15a1a expression at day 3, although the expression did not exceed the pre-fasted level. Observed responses of slc15a1a to fasting and refeeding suggest that the expression level of this gene can serve as a sensitive indicator of the changes that may occur in altering nutritional conditions. These findings contribute to a better understanding of the role of PepT1 in nutrition and of the complex mechanisms underlying the absorption of oligopeptides and amino acids in the intestine, and may lead to development of possible means to manipulate the absorption processes for the improvement of growth and other metabolic and physiological conditions in fish. (C) 2016 Published by Elsevier Inc.