Selected Ion Flow Tube-MS Analysis of Headspace Vapor from Gastric Content for the Diagnosis of Gastro-Esophageal Cancer
Selected Ion Flow Tube-MS Analysis of Headspace Vapor from Gastric Content for the Diagnosis of Gastro-Esophageal Cancer
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DOI：
10.1021/ac302409a
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发表时间：
2012-11-06
影响因子：
7.4
通讯作者：
Hanna, George B.
中科院分区：
文献类型：
--
作者：
Kumar, Sacheen;Huang, Juzheng;Hanna, George B.
胃内容物是人体胃内的复杂生物流体，在消化过程中具有重要作用。据信，胃内容物可能是上消化道疾病发展的一个促成因素。在这项工作中，选择离子流管质谱（SIFT-MS）已被应用到定量的挥发性有机化合物（VOCs）的胃内容物样品，这是从三组患者，包括那些与胃食管癌，非癌症疾病的上胃肠道，和一个健康的队列检索的顶空蒸气。本研究共研究了12种挥发性有机化合物。通过Mann-WhitneyU检验，发现以下7种VOC，丙酮、甲醛、乙醛、己酸、硫化氢、氰化氢和甲基苯酚，在癌症组和健康组之间显著不同。受试者工作特征（ROC）分析应用于乙醛，甲醛，硫化氢和甲基苯酚的组合VOC，以区分癌症患者和健康对照。曲线下面积（AUC）为0.9。这一结果提出了一个前景的VOC档案，而不是一个单一的生物标志物可能是更可取的胃食管癌的分子导向诊断，这值得进一步调查，以评估其潜在的应用作为一个新的诊断测试。
Gastric content is a complex biofluid within the human stomach which has an important role in digestive processes. It is believed that gastric content may be a contributory factor in the development of upper gastro-intestinal diseases. In this work, selected ion flow tube mass spectrometry (SIFT-MS) has been applied to the quantification of volatile organic compounds (VOCs) in the headspace vapor of gastric content samples, which were retrieved from three groups of patients, including those with gastro-esophageal cancer, noncancer diseases of the upper gastro-intestinal tract, and a healthy cohort. Twelve VOCs have been investigated in this study;. the following 7 VOCs, acetone, formaldehyde, acetaldehyde, hexanoic acid, hydrogen sulphide, hydrogen cyanide, and methyl phenol, were found to be significantly different between cancer and healthy groups by the Mann-Whitney U test. Receiver operating characteristics (ROC) analysis was applied for the combined VOCs of acetaldehyde, formaldehyde, hydrogen sulphide, and methyl phenol to discriminate cancer patients from healthy controls. The area under the curve (AUC) was 0.9. This result raises the prospect that a VOC profile rather than a single biomarker may be preferable in the molecular-orientated diagnosis of gastro-oseophageal cancer, and this warrants further investigation to assess its potential application as a new diagnostic test.