Growth ability and immunological properties of Erysipelothrix rhusiopathiae serotype 2.
Growth ability and immunological properties of Erysipelothrix rhusiopathiae serotype 2.
复制标题
猪红斑丹毒丝菌血清型2的生长能力和免疫学特性。
DOI：
10.1292/jvms.58.87
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发表时间：
1996
期刊：
影响因子：
--
通讯作者：
T. Takahashi
中科院分区：
文献类型：
--
作者：
K. Zarkasie;T. Sawada;T. Yoshida;I. Takahashi;T. Takahashi
比较了属于血清型 2 的五种猪红斑丹毒丝菌野外菌株的生长能力、小鼠免疫原性、细胞表面蛋白的 SDS-PAGE 谱及其免疫印迹模式。 Tama-96菌株在Feist培养基和含有Tween 80 (TPB)的胰蛋白胨磷酸肉汤中表现出最稳定的生长，并且在使用由20小时TPB培养物制备的灭活疫苗的小鼠保护试验中其免疫原性最高。该疫苗的50%小鼠保护剂量仅为12微升。总体而言，各菌株中蛋白质的 SDS-PAGE 和免疫印迹模式相似，表明 66 至 64 kDa 的蛋白质抗原占主导地位。
Five field strains of Erysipelothrix rhusiopathiae belonging to serotype 2 were compared for their growth ability, immunogenicity in mice, SDS-PAGE profile of cell surface proteins and their immunoblotting patterns. Strain Tama-96 showed the most stable growth in Feist medium and tryptose phosphate broth with Tween 80 (TPB), and its immunogenicity was highest in a mouse protection test using the inactivated vaccines prepared from 20-h TPB culture. The 50% mouse protective dose of the vaccine was only 12 microliter. SDS-PAGE and immunoblotting patterns of the proteins were similar among the strains in general and indicated that 66 to 64 kDa protein antigens were dominant.