Generation of transgenic mouse line expressing Kusabira Orange throughout body, including erythrocytes, by random segregation of provirus method
Generation of transgenic mouse line expressing Kusabira Orange throughout body, including erythrocytes, by random segregation of provirus method
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DOI：
10.1016/j.bbrc.2013.05.017
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发表时间：
2013-06-14
影响因子：
3.1
通讯作者：
Nakauchi, Hiromitsu
中科院分区：
文献类型：
--
作者：
Hamanaka, Sanae;Ooehara, Jun;Nakauchi, Hiromitsu
转基因小鼠可用于获得标记的体细胞以研究发育或分化的动力学。造血干细胞通常用于研究造血。然而，据我们所知，还没有描述荧光蛋白在所有造血细胞（包括红细胞和血小板）中稳定且组成型表达的转基因小鼠系。利用随机分离前病毒（RSP）方法，从逆转录病毒转导的小鼠胚胎干细胞中获得了表达红色/橙子荧光蛋白Kusabira橙子（KuO）的转基因小鼠品系。KuO转基因小鼠系细胞仅携带一个前病毒整合位点，并在所有造血谱系元素（包括红细胞和血小板）中稳定表达KuO。KuO转基因小鼠骨髓移植后造血功能正常。KuO转基因小鼠可能被证明可用于研究造血，包括红细胞生成和巨核细胞生成。(C)2013 Elsevier Inc. All rights reserved.
Fluorescent-protein transgenic mice are useful for obtaining marked somatic cells to study kinetics of development or differentiation. Fluorescence-marked hematopoietic stem cells in particular are commonly used for studying hematopoiesis. However, as far as we know, no transgenic mouse line is described in which a fluorescent protein is stably and constitutively expressed in all hematopoietic cells, including erythrocytes and platelets. Using the random segregation of provirus (RSP) method, we generated from retrovirally transduced mouse embryonic stem cells a transgenic mouse line expressing a red/orange fluorescent protein, Kusabira Orange (KuO). KuO transgenic mouse line cells carry only one proviral integration site and stably express KuO in all hematopoietic-lineage elements, including erythrocytes and platelets. Moreover, bone-marrow transplantation in KuO transgenic mice demonstrated normal hematopoieisis. KuO transgenic mice likely will prove useful for study of hematopoiesis that includes erythropoiesis and megakaryopoiesis. (C) 2013 Elsevier Inc. All rights reserved.