Transcriptome analysis of the wheat -: Puccinia striiformis f. sp tritici interaction
Transcriptome analysis of the wheat -: Puccinia striiformis f. sp tritici interaction
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DOI：
10.1111/j.1364-3703.2007.00453.x
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发表时间：
2008-03-01
影响因子：
4.9
通讯作者：
Chen, Xianming
中科院分区：
文献类型：
--
作者：
Coram, Tristan E.;Wang, Meinan;Chen, Xianming
条锈病[由条锈病引起的]。p.p tritici Eriks（Pst）]是小麦（Triticum aestivum L.）的一种世界性的破坏性病害。遗传抗性是首选的控制方法，最初在Triticum spelta var. album中发现的Yr5基因是一种主要的抗性(R)基因，在美国对所有已知的Pst小种具有全阶段抗性。为了鉴定与Yr5介导的不相容相互作用和Yr5相容相互作用相关的转录本，利用小麦基因芯片分析了接种Pst后不同Yr5基因存在的小麦等温线发生的变化。这项时间过程研究（接种后6、12、24和48 h）鉴定了在r基因介导的不相容相互作用中诱导的115个转录本，在相容相互作用中诱导的73个转录本。54个转录本在两种相互作用中都被诱导，被认为是基础防御转录本，而61个转录本是不相容相互作用的特异性转录本[过敏反应（HR）特异性转录本]，19个转录本是相容相互作用的特异性转录本（生物营养相互作用特异性转录本）。转录物积累的时间模式显示，在感染后24小时达到峰值，这可能反映了Pst在类似16小时时的吸体渗透。在排除那些被认为是不完全等同源的转录物后，另外12个组成转录物差异归因于Yr5的存在。对诱导转录本的注释显示，Yr5的存在导致了快速和扩增的抗性反应，涉及已知在r基因介导的反应中发生的信号通路和防御相关转录本，包括蛋白激酶信号传导和活性氧的产生，导致超敏反应。基础防御也涉及许多防御相关转录物的大量诱导，但r基因信号的缺乏导致反应较弱。
Stripe rust [caused by Puccinia striiformis Westend. f. sp. tritici Eriks. (Pst)] is a destructive disease of wheat (Triticum aestivum L.) worldwide. Genetic resistance is the preferred method for control and the Yr5 gene, originally identified in Triticum spelta var. album, represents a major resistance (R) gene that confers all-stage resistance to all currently known races of Pst in the United States. To identify transcripts associated with the Yr5-mediated incompatible interaction and the yr5-compatible interaction, the Wheat GeneChip was used to profile the changes occurring in wheat isolines that differed for the presence of the Yr5 gene after inoculation with Pst. This time-course study (6, 12, 24 and 48 h post-inoculation) identified 115 transcripts that were induced during the R-gene-mediated incompatible interaction, and 73 induced during the compatible interaction. Fifty-four transcripts were induced in both interactions and were considered as basal defence transcripts, whilst 61 transcripts were specific to the incompatible interaction [hypersensitive response (HR)-specific transcripts] and 19 were specific to the compatible interaction (biotrophic interaction- specific transcripts). The temporal pattern of transcript accumulation showed a peak at 24 h after infection that may reflect haustorial penetration by Pst at similar to 16 h. An additional 12 constitutive transcript differences were attributed to the presence of Yr5 after eliminating those considered as incomplete isogenicity. Annotation of the induced transcripts revealed that the presence of Yr5 resulted in a rapid and amplified resistance response involving signalling pathways and defence-related transcripts known to occur during R-gene-mediated responses, including protein kinase signalling and the production of reactive oxygen species leading to a hypersensitive response. Basal defence also involved substantial induction of many defence-related transcripts but the lack of R-gene signalling resulted in weaker response.