Metal ion transport quantified by ICP-MS in intact cells.
Metal ion transport quantified by ICP-MS in intact cells.
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DOI：
10.1038/srep20551
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发表时间：
2016-02-03
影响因子：
4.6
通讯作者：
Heiny JA
中科院分区：
文献类型：
--
作者：
Figueroa JA;Stiner CA;Radzyukevich TL;Heiny JA
使用ICP-MS测量生物组织中的金属离子含量为研究金属依赖性生理过程提供了高度灵敏的手段。本文介绍了应用ICP-MS测定小鼠骨骼肌和人红细胞Na，K-ATP酶对Rb和K离子的膜转运。ICP-MS方法提供了比常规示踪剂通量方法更高的精度和统计功效。该方法广泛适用于研究其他金属离子转运蛋白和金属依赖性过程中的一系列细胞类型和条件。
The use of ICP-MS to measure metal ion content in biological tissues offers a highly sensitive means to study metal-dependent physiological processes. Here we describe the application of ICP-MS to measure membrane transport of Rb and K ions by the Na,K-ATPase in mouse skeletal muscles and human red blood cells. The ICP-MS method provides greater precision and statistical power than possible with conventional tracer flux methods. The method is widely applicable to studies of other metal ion transporters and metal-dependent processes in a range of cell types and conditions.