Normal specification of the extraembryonic lineage after somatic nuclear transfer
Normal specification of the extraembryonic lineage after somatic nuclear transfer
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DOI：
10.1016/j.febslet.2006.02.036
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发表时间：
2006-03-20
期刊：
影响因子：
3.5
通讯作者：
Wakayama, T
中科院分区：
文献类型：
--
作者：
Kishigami, S;Hikichi, T;Wakayama, T
为了探讨滋养外胚层(TE)系在体细胞克隆胚泡中的建立和维持，采用免疫组织化学方法同时检测了决定TE命运的关键分子CDX2的表达和Oct4的表达。克隆的小鼠胚胎是用卵丘细胞、尾尖成纤维细胞和胚胎干细胞进行核移植制成的。培养96h后，卵丘细胞克隆和成纤维细胞克隆的Oct4表达囊胚率分别为50%和60%。然而，无论Oct4的表达如何，大多数克隆的囊胚(>；90%)正常表达CDX2。因此，即使体细胞克隆胚胎减少了产生内细胞团谱系的可能性，TE谱系仍然可以建立和维持。(C)2006年欧洲生化学会联合会。爱思唯尔出版，版权所有。
To examine the establishment and maintenance of trophectoderm (TE) lineage in somatic cloned blastocysts, the expression of Cdx2, a key molecule for specification of TE fate, was immunohistochemically examined simultaneously with Oct4 expression. Cloned mouse embryos were made by nuclear transfer using cumulus cells, tail-tip fibroblasts, and embryonic stem cells. After 96 h of culture, the rates of Oct4-expressmg blastocysts were as low as 50%, and 60% for cumulus and fibroblast clones, respectively. However, regardless of Oct4 expression, the majority of those cloned blastocysts (> 90%) normally expressed Cdx2. Thus, even though somatic cloned embryos have reduced potential to produce the inner cell mass lineage, the TE lineage can be established and maintained. (c) 2006 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.