A C-13 COMPARATIVE NUCLEAR MAGNETIC-RESONANCE STUDY OF ORGANIC SOLUTE PRODUCTION AND EXCRETION BY THE YEASTS HANSENULA-ANOMALA AND SACCHAROMYCES-CEREVISIAE IN SALINE MEDIA
A C-13 COMPARATIVE NUCLEAR MAGNETIC-RESONANCE STUDY OF ORGANIC SOLUTE PRODUCTION AND EXCRETION BY THE YEASTS HANSENULA-ANOMALA AND SACCHAROMYCES-CEREVISIAE IN SALINE MEDIA
复制标题
DOI：
10.1139/m88-100
复制
发表时间：
1988-05-01
影响因子：
2.8
通讯作者：
LARHER, F
中科院分区：
文献类型：
--
作者：
BELLINGER, Y;LARHER, F
甘油、阿拉伯糖醇和海藻糖是使用自然丰度 13C 核磁共振波谱在异常汉逊酵母细胞提取物中检测到的主要溶质。只有两种多元醇随着盐度的增加而积累，甘油的增加要大得多。阿拉伯糖醇含量也随着培养年龄而增加，与盐的存在或不存在无关，并且与海藻糖含量的演变一致。汉逊酵母的甘油保留潜力是酿酒酵母的 15 倍。前者表现出在高盐浓度下增加这种容量的特殊性质。在这种条件下，相对于对盐敏感的参考酵母酿酒酵母，其生长与单位生物量葡萄糖消耗的有限增加有关。此外，仅在存在盐的汉逊酵母的外部培养基中检测到一种多糖，其化学性质尚未进一步表征。
Glycerol, arabitol and trehalose were the principle solutes detected in cellular extracts of Hansenula anomala, using natural-abundance 13C nuclear magnetic resonance spectroscopy. Only the two polyols accumulated in response to increased salinity, glycerol increase being far greater. Arabitol content also increased with culture age, independently of the presence or absence of salt and in line with the evolution of threhalose content. Glycerol retention potential was 15 times greater for Hansenula than for Saccharomyces cerevisiae. The former displayed the specific property of increasing this capacity in high salt concentrations. Under such conditions its growth was associated with a limited increase in glucose consumption per unit biomass, relative to S. cerevisiae, the salt-sensitive reference yeast. In addition, a polysaccharide, the chemical nature of which was not further characterized, was detected exclusively in the external medium of Hansenula growing in the presence of salt.