In vivo pump-probe optical coherence tomography imaging in Xenopus laevis.
In vivo pump-probe optical coherence tomography imaging in Xenopus laevis.
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DOI：
10.1002/jbio.201300119
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发表时间：
2015-01
影响因子：
2.8
通讯作者：
Applegate BE
中科院分区：
文献类型：
--
作者：
Carrasco-Zevallos O;Shelton RL;Kim W;Pearson J;Applegate BE
Currently, optical coherence tomography (OCT), is not capable of obtaining molecular information often crucial for identification of disease. To enable molecular imaging with OCT, we have further developed a technique that harnesses transient changes in light absorption in the sample to garner molecular information. A Fourier-domain Pump-Probe OCT (PPOCT) system utilizing a 532 nm pump and 830 nm probe has been developed for imaging hemoglobin. Methylene blue, a biological dye with well-know photophysics, was used to characterize the system before investigating the origin of the hemoglobin PPOCT signal. The first in vivo PPOCT images were recorded of the vasculature in Xenopus laevis. The technique was shown to work equally well in flowing and non-flowing vessels. Furthermore, PPOCT was compared with other OCT extensions which require flow, such as Doppler OCT and phase-variance OCT. PPOCT was shown to better delineate tortuous vessels, where nodes often restrict Doppler and phase-variance reconstruction.
