The AngR protein and the siderophore anguibactin positively regulate the expression of iron-transport genes in Vibrio anguillarum
The AngR protein and the siderophore anguibactin positively regulate the expression of iron-transport genes in Vibrio anguillarum
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DOI：
10.1111/j.1365-2958.1996.tb02662.x
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发表时间：
1996-10-01
影响因子：
3.6
通讯作者：
Crosa, JH
中科院分区：
文献类型：
--
作者：
Chen, Q;Wertheimer, AM;Crosa, JH
鳗弧菌的毒力与铁限制条件下表达的质粒介导的铁摄取系统的存在有关，该系统由铁载体鳗毒素和特定的铁转运蛋白组成。该系统在铁限制条件下最大限度地表达，并且需要AngR蛋白，该AngR蛋白充当anguibactin生物合成的正调节剂，并且还具有可能在anguibactin生物合成中起作用的EntE样酶功能。在这项工作中，我们证明，除了拥有这些相关的anguibactin生产功能，AngR也积极调节铁转运基因fatA和fatB的转录。我们还表明，转录的angR被抑制富铁条件下的毛皮。此外，我们提出的证据表明，anguibactin本身增强铁转运基因fatA和fatB的转录，独立于AngR和反式作用因子（TAF）的产品（S）。AngR（与TAF产物一起）或anguibactin单独存在会导致铁转运基因fatA和fatB的部分表达，而当AngR、TAF产物和anguibactin全部存在时，则实现完全表达。
Vibrio anguillarum virulence is associated with the presence of a plasmid-mediated iron-uptake system expressed under iron-limiting conditions, which consists of the siderophore anguibactin and specific iron-transport proteins. This system is maximally expressed under iron-limiting conditions and requires the AngR protein that acts as a positive regulator of anguibactin biosynthesis and also possess an EntE-like enzymatic function that may play a role in anguibactin biosynthesis. In this work, we demonstrate that in addition to possessing these functions related to anguibactin production, AngR also positively regulated transcription of the iron-transport genes fatA and fatB. We also show that transcription of angR is repressed by Fur under iron-rich conditions. In addition, we present evidence that anguibactin itself enhanced transcription of the iron-transport genes fatA and fatB, independently of AngR and the trans-acting factor (TAF) product(s). The presence of either AngR (together with the TAF product(s)) or anguibactin alone led to a partial level of expression of the iron-transport genes fatA and fatB, while full expression is achieved when AngR, the TAF products and anguibactin are all present.