MAPPING AND CHARACTERIZATION OF AN X-LINKED PROCESSED GENE RELATED TO MYCL1
MAPPING AND CHARACTERIZATION OF AN X-LINKED PROCESSED GENE RELATED TO MYCL1
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DOI：
10.1016/0888-7543(89)90344-3
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发表时间：
1989-04-01
期刊：
影响因子：
4.4
通讯作者：
SHOWS, TB
中科院分区：
文献类型：
--
作者：
MORTON, CC;NUSSENZWEIG, MC;SHOWS, TB
与原癌基因的myc家族同源的DNA序列已被表征并发现是与L-MYC（MYCL 1）相关的加工基因。通过与从myc基因家族的高度保守区域中的c-MYC（MYC）序列合成的寡核苷酸探针交叉杂交来分离该加工基因（MYCL 2）。序列分析表明，MYCL 2的开放阅读框为1194 bp，与最近发表的MYCL 1的DNA序列有很强的同源性。Southern和北方杂交分析证实了MYCL 1与小细胞肺癌的同源性。通过体细胞杂种对MYCL 2作图将该序列置于X染色体长臂上的q22 →条带中。问题28。
A DNA sequence with homology to the myc family of proto-oncogenes has been characterized and found to be a processed gene related to L-MYC (MYCL1). This processed gene (MYCL2) was isolated by cross-hybridization to an oligonucleotide probe synthesized from the c-MYC (MYC) sequence in a highly conserved region of the myc gene family. Sequence analysis of MYCL2 revealed an open reading frame of 1194 bp with no intervening sequences and strong homology to the recently published DNA sequence of MYCL1. Southern and Northern blot analyses of DNAs and RNAs from small cell lung carcinoma confirmed its MYCL1 homology. Mapping of MYCL2 by somatic cell hybrids places this sequence on the long arm of the X chromosome in bands q22 .fwdarw. q28.