Identification and characterization of an MGSA/GRO pseudogene.
Identification and characterization of an MGSA/GRO pseudogene.
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DOI：
10.3109/10425179709034060
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发表时间：
1997
期刊：
影响因子：
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通讯作者：
R. Shattuck-Brandt;L. Wood;A. Richmond
中科院分区：
文献类型：
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作者：
R. Shattuck-Brandt;L. Wood;A. Richmond
CXC趋化因子黑色素瘤生长刺激活性/生长相关蛋白（MGSA/GRO）的三个连锁基因先前已被表征并定位于染色体4 q12-q13。我们已经分离并鉴定了假基因MGSA/GRO delta，其在跨越5' UTR、第一和第二外显子以及第一内含子的区域中与MGSA/GRO alpha基因具有83%的相似性。MGSA/GRO δ基因的5'上游序列也与MGSA/GRO α、β、γ基因非常相似，含有保守的NF-κ B基序、TATA盒和转录起始位点。然而，该序列在第二个外显子之后变得明显不同，并且杂交研究表明与其他MGSA/GRO基因的第三和第四个外显子相似的序列不存在于该基因中。其他序列差异包括MGSA/GRO δ翻译起始密码子的改变和一个碱基插入，导致外显子2内的明显移码和早期终止。像这样的多重突变是假基因的特征。
Three linked genes for the CXC-chemokine melanoma growth stimulatory activity/growth related protein (MGSA/GRO) have been previously characterized and mapped to chromosome 4q12-q13. We have isolated and characterized a pseudogene, MGSA/GRO delta, which is 83% similar to the MGSA/GRO alpha gene in the region spanning the 5' UTR, first and second exons, and the first intron. The 5' upstream sequence for the MGSA/GRO delta gene, which is also very similar to the MGSA/GRO alpha, beta, gamma genes, contains a conserved NF-kappa B motif, a TATA box, and a transcription initiation site. However, the sequence becomes markedly divergent after the second exon and hybridization studies indicate that sequences similar to the third and forth exons of other MGSA/GRO genes are not present in this gene. Additional sequence differences include alteration of the MGSA/GRO delta translation initiation codon and a one base insertion resulting in an apparent frame shift and early termination within exon 2. Multiple mutations such as these are characteristic of pseudogenes.