Gene and protein profiling of the response of MA-10 Leydig tumor cells to human chorionic gonadotropin.
Gene and protein profiling of the response of MA-10 Leydig tumor cells to human chorionic gonadotropin.
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MA-10 Leydig 肿瘤细胞对人绒毛膜促性腺激素反应的基因和蛋白质分析。
DOI：
10.1002/j.1939-4640.2004.tb03160.x
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发表时间：
2004
期刊：
影响因子：
--
通讯作者：
Papadopoulos,Vassilios
中科院分区：
文献类型：
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作者：
Li,Wenping;Amri,Hakima;Huang,Hongzhan;Wu,Cathy;Papadopoulos,Vassilios
肽激素激活甾体生成机制涉及多个步骤，以空间和时间协调的方式将信号从质膜传递到线粒体。虽然已经确定了介导激素信号的关键蛋白，但最近的数据表明，该途径可能涉及更复杂的蛋白-蛋白和蛋白-脂质的相互作用。基因组学和蛋白质组学分析方法，即Affymetrix小鼠基因组U74A v2基因芯片和BD PowerBlot Western阵列，用于鉴定人绒毛膜促性腺激素（hCG）诱导的MA - 10 Leydig肿瘤细胞mRNA和蛋白的变化，这些变化与孕激素合成的增加平行。为了分析产生的大量数据，开发了一个综合的蛋白质信息矩阵，总结了每个基因或蛋白质的特征，包括其已知特性，以及基于同源性的功能推断得出的注释。在Affymetrix阵列检测的基因中，约有79个基因存在差异表达，而在PowerBlot检测的基因产物中，有9个基因存在差异表达（以上两倍）。使用实时定量聚合酶链反应和免疫印迹分析证实了所选转录本表达的变化。总之，这些结果表明激素调节类固醇形成是一个复杂的现象，涉及到参与各种已知和新的途径的蛋白质，这些途径涉及从质膜到线粒体和细胞核的信号传递。
Activation of the steroidogenic machinery by peptide hormones involves a number of steps for transmitting signals from the plasma membrane to mitochondria in a spatially and temporally coordinated manner. Although key proteins mediating the hormonal signal have been identified, recent data suggest that the pathway might involve more complex protein‐protein and protein‐lipid interactions. Genomic and proteomic methods of analysis, namely the Affymetrix Murine Genome U74A v2 GeneChip and the BD PowerBlot Western Array, were used to identify human chorionic gonadotropin (hCG)‐induced changes in mRNA and protein of MA‐10 Leydig tumor cells that parallel the increase seen in progesterone synthesis. To analyze the massive amount of data that was generated, a comprehensive protein information matrix summarizing the features of each gene or protein, including its known properties, as well as annotations derived by homology‐based functional inference, was developed. Of the genes examined by Affymetrix array, approximately 79 were differentially expressed and of gene products examined by PowerBlot, 9 were differentially expressed (above twofold). Changes in the expression of selected transcripts of interest were confirmed using real‐time quantitative polymerase chain reaction and immunoblot analyses. Collectively, these results indicate that hormonal regulation of steroidogenesis is a complex phenomenon, involving proteins that participate in various known and novel pathways, which are implicated in transmitting signals from the plasma membrane to mitochondria and nucleus.