Human insulin receptor radioimmunoassay: applicability to insulin-resistant states.
Human insulin receptor radioimmunoassay: applicability to insulin-resistant states.
复制标题
人胰岛素受体放射免疫测定：对胰岛素抵抗状态的适用性。
DOI：
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发表时间：
1989
影响因子：
--
通讯作者：
I. Goldfine
中科院分区：
文献类型：
--
作者：
V. Pezzino;V. Papa;V. Trischitta;A. Brunetti;P. Goodman;M. Treutelaar;J. Williams;B. Maddux;R. Vigneri;I. Goldfine
人胰岛素受体的放射免疫测定法的开发，采用有效的兔多克隆抗体的人胰岛素受体和高度纯化的人胎盘胰岛素受体制剂。用胰岛素受体单克隆抗体-琼脂糖和麦胚凝集素-琼脂糖连续亲和层析获得受体，用125 I-Bolton-Hunter试剂以2，100 - 3，300 Ci/mmol的比活度进行放射性标记。超过75%的这种配体与多克隆抗受体抗体免疫沉淀，并保持免疫沉淀大于45天。该测定对低至0.2 ng/0.5 ml的未标记受体浓度敏感;未标记胰岛素不发生交叉反应，未标记胰岛素样生长因子（IGF）-I受体交叉反应较弱。放射免疫测定法适用于测定组织和细胞中的胰岛素受体，这些受体是通过在1%Triton X-100中增溶提取的;不需要纯化提取的受体。在研究的胰岛素作用的三个主要靶组织中，肝脏的受体浓度最高（47.6 ng/mg蛋白质）;脂肪和肌肉的水平较低。放射免疫分析的其他研究表明，在肥胖高胰岛素血症受试者的单核细胞和妖精病患者的成纤维细胞中，胰岛素受体均减少。
A radioimmunoassay of the human insulin receptor was developed employing a potent rabbit polyclonal antibody to the human insulin receptor and a highly purified human placental insulin receptor preparation. The receptor, obtained by sequential affinity chromatography with insulin receptor monoclonal antibody-agarose and wheat germ agglutinin-agarose, was radiolabeled with 125I-Bolton-Hunter reagent at specific activities of 2,100-3,300 Ci/mmol. Over 75% of this ligand was immunoprecipitable with the polyclonal antireceptor antibody and remained immunoprecipitable for greater than 45 days. The assay was sensitive to unlabeled receptor concentrations as low as 0.2 ng/0.5 ml; unlabeled insulin did not cross-react and unlabeled insulin-like growth factor (IGF)-I receptor cross-reacted weakly. The radioimmunoassay was applicable to the measurement of insulin receptors in tissues and cells that were extracted by solubilization in 1% Triton X-100; no purification of the extracted receptor was necessary. Of the three major target tissues for insulin action studied, liver had the highest concentration of receptors (47.6 ng/mg protein); fat and muscle had lower levels. Other studies with the radioimmunoassay indicated that insulin receptors were decreased both in monocytes from obese hyperinsulinemic subjects and in fibroblasts from patients with leprechaunism.
胰岛素受体的固相竞争性放射免疫测定。
影响因子：
2.9
作者：
Roth,RA;Beaudoin,J
通讯作者：
Beaudoin,J
小妖精患者成纤维细胞中胰岛素受体的功能。
DOI：
10.1172/jci113739
发表时间：
1988
期刊：
The Journal of clinical investigation
影响因子：
--
作者：
Reddy,SS;Lauris,V;Kahn,CR
通讯作者：
Kahn,CR