Independent circadian regulation of assimilation and stomatal conductance in the ztl-1 mutant of Arabidopsis
Independent circadian regulation of assimilation and stomatal conductance in the ztl-1 mutant of Arabidopsis
复制标题
DOI：
10.1111/j.1469-8137.2004.01005.x
复制
发表时间：
2004-04-01
期刊：
影响因子：
9.4
通讯作者：
Webb, AAR
中科院分区：
文献类型：
--
作者：
Dodd, AN;Parkinson, K;Webb, AAR
我们已经开发了一个低成本，多比色皿红外气体分析仪系统的中期测量拟南芥植物的气体交换。该系统能够并行测量气孔导度和CO2同化，提供基因型之间的直接比较。这使得分子遗传学工具，可在拟南芥结合的权力，整个植物生理学。该系统是专门设计的定量昼夜节律的气体交换，并控制比色皿的相对湿度和CO2浓度在连续光照和光暗周期。比色皿的湿度由一个独特的反馈系统控制，防止比色皿内的湿度振荡。使用这个新系统，我们证明，在连续光照下，zeitlupe（ztl-1）突变体中的气孔导度和CO2固定的昼夜节律周期比野生型长。因此，野生型ZTL表达是CO2固定和气孔导度正常循环所必需的。此外，我们证明了不同的昼夜节律周期可以共存于一个单一的植物，突出了植物昼夜节律振荡器的细胞自主性。
We have developed a low-cost, multicuvette infrared gas analyser system for medium-term measurement of gas exchange in Arabidopsis thaliana plants. The system enables parallel measurement of stomatal conductance and CO2 assimilation, providing direct comparison between genotypes. This allows the molecular genetic tools that are available in Arabidopsis to be combined with the power of whole-plant physiology.The system was designed specifically for quantification of circadian rhythms of gas exchange, and controls cuvette relative humidity and CO2 concentration under both continuous light and light-dark cycles. Cuvette humidity is controlled by a unique feedback system that prevents humidity oscillations within the cuvettes.Using this new system we demonstrated that, under continuous light, there was a longer circadian period for both stomatal conductance and CO2 fixation in the zeitlupe (ztl-1) mutant compared to wild type.Wild-type ZTL expression is therefore required for normal cycles of CO2 fixation and stomatal conductance. Furthermore, we demonstrate that different circadian periods can coexist in a single plant, highlighting the cell autonomous nature of the plant circadian oscillator.