A highly sensitive and specific chemiluminescent enzyme-linked immunosorbent assay for diagnosis of active Trypanosoma cruzi infection
A highly sensitive and specific chemiluminescent enzyme-linked immunosorbent assay for diagnosis of active Trypanosoma cruzi infection
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DOI：
10.1046/j.1537-2995.1997.37897424410.x
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发表时间：
1997-08-01
期刊：
影响因子：
2.9
通讯作者：
Travassos, LR
中科院分区：
文献类型：
--
作者：
Almeida, IC;Covas, DT;Travassos, LR
背景技术背景：查加斯病通过携带克氏锥虫的昆虫叮咬或通过受感染献血者的输血传播给人类。目前在南美洲血库中使用的常规血清学检测是不令人满意的，因为大量的不确定和假阳性结果。其他方法，如聚合酶链反应和酶联免疫吸附测定（ELISA）与重组抗原已被提出，但固有的困难，迄今已排除了他们通过在大规模的筛选所需的bloodbanks.Study设计和方法：一个高度敏感和特异性的荧光ELISA使用纯化的锥鞭虫糖缀合物抗原和复杂的epimastigote抗原被设计用于诊断活动性T。克氏感染结果：化学发光ELISA对100例确诊的南美锥虫病的诊断敏感性为100%。在一组115份血清中排除了不确定的结果和假阳性反应。对1000份正常人血清和288份其他感染（包括利什曼病）患者和免疫接种者的异源血清进行检测，用纯化的锥鞭虫糖缀合物抗原和复合外鞭毛体抗原的特异性分别为100%和99.7%。荧光ELISA提供了一种高度灵敏的检测方法，（纯化的锥鞭虫糖缀合物和复合表鞭虫抗原）和特异性的（纯化的锥鞭虫糖缀合物抗原）用于恰加斯病诊断。它可用于血库筛查和监测接受化疗的患者的治疗。
BACKGROUND: Chagas' disease is transmitted to man either by the bite of insects harboring Trypanosoma cruzi or by the transfusion of blood from infected donors. The conventional serologic testing as presently used in blood banks in South America is unsatisfactory, because of a high number of inconclusive and false-positive results. Other methods such as polymerase chain reaction and enzyme-linked immunosorbent assay (ELISA) with recombinant antigens have been proposed, but inherent difficulties have so far precluded their adoption in the large-scale screening required by blood banks.STUDY DESIGN AND METHODS: A highly sensitive and specific chemiluminescent ELISA using a purified trypomastigote glycoconjugate antigen and a complex epimastigote antigen was devised for the diagnosis of active T. cruzi infection. RESULTS: Chemiluminescent ELISA was 100-percent sensitive in the diagnosis of 100 cases of confirmed Chagas' disease. Inconclusive results and false-positive reactions were eliminated in a panel of 115 sera. The specificity of the chemiluminescent ELISA was 100 percent with a purified trypomastigote glycoconjugate antigen and 99.7 percent wiih a complex epimastigote antigen when applied to 1000 normal human sera and 288 heterologous sera from patients with other infections, including leishmaniasis, and vaccinated individuals.CONCLUSION: The chemiluminescent ELISAs provide a test that is highly sensitive (purified trypomastigote glycoconjugate and complex epimastigote antigens) and specific (purified trypomastigote glycoconjugate antigen) for Chagas' disease diagnosis. It can be used in blood bank screening and to monitor the treatment of patients undergoing chemotherapy.