INTERLEUKIN-6 INDUCES A LIVER-SPECIFIC NUCLEAR-PROTEIN THAT BINDS TO THE PROMOTER OF ACUTE-PHASE GENES
INTERLEUKIN-6 INDUCES A LIVER-SPECIFIC NUCLEAR-PROTEIN THAT BINDS TO THE PROMOTER OF ACUTE-PHASE GENES
复制标题
DOI：
10.1073/pnas.86.21.8202
复制
发表时间：
1989-11-01
影响因子：
11.1
通讯作者：
CORTESE, R
中科院分区：
文献类型：
--
作者：
POLI, V;CORTESE, R
白细胞介素 6 (IL-6) 负责与激活感染或炎症防御相关的多种生物效应，包括免疫反应和急性期反应。其作用机制尚不清楚。最近显示它可以诱导编码急性期蛋白的几种基因的转录。在这里，我们描述了人血红素基因启动子中存在的 IL-6 反应元件 (IL-6RE) 的鉴定。该元件对于 IL-6 依赖性转录激活是必要且充分的。 IL-6 效应不需要从头合成蛋白质。肝脏特异性核蛋白 (IL-6DBP) 与血红素结合蛋白 IL-6RE 以及其他急性期基因启动子上的类似序列结合。 IL-6DBP DNA 结合活性由 IL-6 通过翻译后机制诱导。
Interleukin 6 (IL-6) is responsible for a variety of biological effects related to the activation of defenses against infection or inflammation, including the immune response and the acute-phase reaction. Its mechanism of action is unknown. It has recently been shown to induce transcription of several genes encoding acute-phase proteins. Here we describe the identification of an IL-6 responsive element (IL-6RE) present in the promoter of the human hemopexin gene. This element is necessary and sufficient for the IL-6-dependent activation of transcription. The IL-6 effect does not require de novo protein synthesis. A liver-specific nuclear protein (IL-6DBP) binds to the hemopexin IL-6RE as well as to similar sequences on the promoter of other acute-phase genes. IL-6DBP DNA binding activity is induced by IL-6 via a posttranslational mechanism.