Tsetse Fly Transmission Studies of African Trypanosomes.

Tsetse Fly Transmission Studies of African Trypanosomes.
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非洲锥虫的采采蝇传播研究。

DOI:
10.1007/978-1-0716-0294-2_4
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发表时间:
2020
期刊:
Methods in molecular biology (Clifton, N.J.)
影响因子:
--
通讯作者:
Peacock L
Peacock L
中科院分区:
--
文献类型:
--
作者:
Peacock L

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非洲锥虫是通过撒哈拉以南非洲的吸血采采蝇自然传播的,如果有干净的采采蝇和合适的锥虫用于实验,这些传播周期可以在实验室中复制。采采虫传播可获得比体外培养更多的锥虫发育阶段,尽管数量很少;例如,布鲁氏锥虫的有性阶段已从感染采采蝇的唾液腺中分离出来,但尚未从培养中报告。采采素的传播还可以研究不同发育阶段之间的自然过渡。野生型和转基因锥虫都已成功地传播到苍蝇体内,并有可能在一定程度上操纵苍蝇体内的锥体环境,例如通过给苍蝇喂食四环素来诱导受Tet阻滞物控制的基因的表达。
African trypanosomes are naturally transmitted by bloodsucking tsetse flies in sub-Saharan Africa and these transmission cycles can be reproduced in the laboratory if clean tsetse flies and suitable trypanosomes are available for experiments. Tsetse transmission gives access to more trypanosome developmental stages than are available from in vitro culture, albeit in very small numbers; for example, the sexual stages ofTrypanosoma bruceihave been isolated from infected tsetse salivary glands, but have not yet been reported from culture. Tsetse transmission also allows for the natural transition between different developmental stages to be studied.Both wild-type and genetically modified trypanosomes have been successfully fly transmitted, and it is possible to manipulate the trypanosome environment inside the fly to some extent, for example, the induction of expression of genes controlled by the Tet repressor by feeding flies with tetracycline.
舌蝇中间日锥虫和刚果锥虫的食虫感染
DOI: --
发表时间: 2004
影响因子: 2
作者:
D. Jefferies;M. Helfrich;D. Molyneux
通讯作者: D. Molyneux
在没有活宿主的情况下大规模饲养采采蝇(舌蝇属)。
DOI: --
发表时间: 1977
期刊:
影响因子: --
作者:
A. Mews;P. Langley;R. W. Pimley;M. Flood
通讯作者: M. Flood