A real-time simultaneous measurement on a microfluidic device for individual bacteria discrimination
A real-time simultaneous measurement on a microfluidic device for individual bacteria discrimination
复制标题
DOI：
10.1016/j.snb.2018.01.079
复制
发表时间：
2018-05-01
影响因子：
8.4
通讯作者：
Baba, Yoshinobu
中科院分区：
文献类型：
--
作者：
Yasaki, Hirotoshi;Yasui, Takao;Baba, Yoshinobu
快速检测病原菌是预防和确定影响人类健康问题的原因的重要社会问题之一。通过孔的离子电流传感已显示出对细菌进行电学测量的能力。然而，现有的离子电流传感方法在区分细菌的化学特性方面存在固有局限性。在此，我们提出一种实时同步测量方法，该方法在微流控装置上结合了离子电流传感和荧光观察。我们的方法不仅可以检测通过微孔的单个细菌的大小，还能在300毫秒内根据细菌细胞表面的化学性质观察其染色性。我们通过结合能够检测70纳米尺寸差异的高精度离子电流传感以及能够区分诸如将细菌定义为革兰氏阳性或革兰氏阴性等内部结构的荧光观察，成功区分了包含污染物颗粒的混合溶液中的每种细菌。我们的方法不仅可应用于现场细菌检测，还可应用于筛选技术。(C) 2018作者。由爱思唯尔出版集团出版。
Rapid detection of pathogenic bacteria is one of the important social issues for preventing and identifying cause of problems affecting human health. Ionic current sensing through pores has shown the ability to electrically measure bacteria. However, there is an inherent limitation to discriminate chemical characteristics of bacteria for existing ionic current sensing methods Here we propose a real-time simultaneous measurement method, which combines ionic current sensing and fluorescence observation on a microfluidic device. Our method can detect not only the size of individual bacteria passing through a micropore but also observe their stainability based on chemical properties of bacterial cell surface within 300 ms. We succeeded in discriminating each bacterium in a solution mixture including contaminant particles by combining highly accurate ionic current sensing which can detect a size difference of 70 nm, and fluorescence observation which can discriminate internal structures such as those that define bacteria as gram-positive or gram-negative. Our method can be applied to not only on-site bacteria detection but also to screening technology. (C) 2018 The Authors. Published by Elsevier B.V.