Release of adenylate kinase 2 from the mitochondrial intermembrane space during apoptosis
Release of adenylate kinase 2 from the mitochondrial intermembrane space during apoptosis
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DOI：
10.1016/s0014-5793(99)00251-3
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发表时间：
1999-03-19
期刊：
影响因子：
3.5
通讯作者：
Zhivotovsky, B
中科院分区：
文献类型：
--
作者：
Köhler, C;Gahm, A;Zhivotovsky, B
细胞色素 c 和凋亡诱导因子 (AIF) 两种线粒体蛋白释放到正在凋亡的细胞的可溶性细胞质中的情况已得到充分证实。使用分光光度法测定酶活性，最近还观察到凋亡细胞胞质部分中腺苷酸激酶（AK）活性的积累。然而，三种同工酶 AK1、AK2 和 AK3 已在哺乳动物细胞中得到表征，并显示分别定位于细胞质、线粒体膜间隙和线粒体基质中，并且尚不清楚这些同工酶中哪一种在细胞凋亡过程中积累在细胞质中。我们现在证明，在凋亡细胞中，只有 AK2 易位到细胞质中。 细胞质中与细胞色素 c 相伴，细胞质中 AK1 的量以及基质相关 AK3 的量在细胞凋亡过程中保持不变。因此，我们的数据表明，在细胞凋亡过程的早期阶段，只有膜间蛋白从线粒体中释放。 (C) 1999 年欧洲生化学会联合会。
The release of two mitochondrial proteins, cytochrome c and apoptosis-inducing factor (AIF), into the soluble cytoplasm of cells undergoing apoptosis is well established. Using spectrophotometric determination of enzyme activity, the accumulation of adenylate kinase (AK) activity in the cytosolic fraction of apoptotic cells has also been observed recently. However, three isozymes, AK1, AK2 and AK3, have been characterized in mammalian cells and shown to be localized in the cytosol, mitochondrial intermembrane space and mitochondrial matrix, respectively, and it is unknown which one of these isozymes accumulates in the cytosol during apoptosis, We now demonstrate that in apoptotic cells only AK2 was translocated into the cytosol concomitantly with cytochrome c, The amount of AK1 in cytosol, as well as the amount of matrix-associated AK3, remained unchanged during the apoptotic process. Thus, our data suggest that only intermembrane proteins are released from mitochondria during the early phase of the apoptotic process. (C) 1999 Federation of European Biochemical Societies.