IMMUNOCHEMICAL STUDIES OF TOBACCO MOSAIC-VIRUS .6. ATTEMPTS TO LOCALIZE VIRAL EPITOPES WITH MONOCLONAL-ANTIBODIES
IMMUNOCHEMICAL STUDIES OF TOBACCO MOSAIC-VIRUS .6. ATTEMPTS TO LOCALIZE VIRAL EPITOPES WITH MONOCLONAL-ANTIBODIES
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DOI：
10.1016/0161-5890(85)90169-5
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发表时间：
1985-01-01
影响因子：
3.6
通讯作者：
VANREGENMORTEL, MHV
中科院分区：
文献类型：
--
作者：
ALTSCHUH, D;MOUDALLAL, ZA;VANREGENMORTEL, MHV
通过测定18株针对烟草花叶病毒(TMV)的单抗与病毒突变体、其他烟草花叶病毒、病毒解离亚基和病毒外壳蛋白多肽片段的结合能力，研究了18株抗TMV单抗的特异性。抗体的表观结合特异性取决于所使用的酶联免疫吸附试验的类型，可能是因为抗原在附着在微滴定孔的塑料表面时被分散或变性。用不同的单抗检测病毒外壳蛋白中的单个替换的能力来描述TMV的一些拓扑表位。通过计算机生成的病毒亚单位表面残基的图像，可以识别与某些单抗结合所涉及的某些残基簇。这些结果清楚地说明了当使用单抗来阐明蛋白质的抗原结构时所遇到的操作限制。
The specificity of 18 monoclonal antibodies directed to tobacco mosaic virus (TMV) was studied by measuring their ability to bind to viral mutants, to other tobamoviruses, to dissociated viral subunits and to peptide fragments of the viral coat protein. The apparent binding specificity of the antibodies was dependent on the type of enzyme-linked immunosorbent assay [ELISA] used, probably because the antigens were distrupted or denatured when attached to the plastic surface of microtiter wells. The capacity of different monoclonal antibodies to detect single substitutions in the viral coat protein was used to delineate some of the topographic epitopes of TMV. By means of computer-generated images of the surface residues of the viral subunit, it was possible to identify certain clusters of residues involved in binding to some of the monoclonal antibodies. The results clearly illustrate the operational limitations encountered when monoclonal antibodies are used for elucidating the antigenic structure of proteins.