Choline acetyltransferase immunoreactivity in the human small and large intestine
Choline acetyltransferase immunoreactivity in the human small and large intestine
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DOI：
10.1053/gast.1996.v111.pm8690205
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发表时间：
1996-08-01
期刊：
影响因子：
29.4
通讯作者：
Costa, M
中科院分区：
文献类型：
--
作者：
Porter, AJ;Wattchow, DA;Costa, M
背景与目的：胆碱乙酰转移酶是参与乙酰胆碱合成的酶，是胆碱能神经元的标志物。在这项研究中，胆碱乙酰转移酶的免疫反应性在人体肠道的分布。研究方法：制备了人小肠和大肠的冰冻切片和整体标本，并用胆碱乙酰转移酶抗血清标记。双标记与抗血清神经元特异性烯醇化酶使所有神经元的胆碱乙酰转移酶的免疫反应的比例被确定。结果如下：神经纤维，胆碱乙酰转移酶免疫反应，经常在环形和纵向肌肉层，并广泛分布在肌间和粘膜下神经丛，但没有观察到在粘膜。肌间神经元，胆碱乙酰转移酶的免疫反应，表现出各种形态，最常见的是单极，有几个短，板层过程的不规则轮廓。64%的肌间神经元胆碱乙酰转移酶免疫反应。粘膜下胆碱能神经元形态均一，胞体椭圆形，胞体光滑，树突呈丝状，占粘膜下神经元总数的53%。小肠和大肠上皮中的一些类似肠内分泌细胞的细胞具有强烈的胆碱乙酰转移酶免疫反应性。结论：人小肠和大肠的大部分神经元是胆碱能神经元。
Background & Aims: Choline acetyltransferase, an enzyme involved in the synthesis of acetylcholine, is a marker of cholinergic neurons. In this study, the distribution of choline acetyltransferase immunoreactivity in human intestine is described. Methods: Frozen-section and whole-mount preparations of human small and large bowels were made and labeled with antiserum to choline acetyltransferase. Double labeling with antiserum to neuron-specific enolase enabled the proportion of all neurons that were immunoreactive for choline acetyltransferase to be determined. Results: Nerve fibers, immunoreactive for choline acetyltransferase, were frequent in the circular and longitudinal muscle layers and were widespread in the myenteric and submucous plexuses, but none was observed in the mucosa. Myenteric neurons, immunoreactive for choline acetyltransferase, showed various morphologies, the most common being unipolar and having an irregular outline with several short, lamellar processes. Sixty-four percent of all myenteric neurons were immunoreactive for choline acetyltransferase. Cholinergic submucous neurons were homogeneous in appearance with oval, smooth cell bodies and filamentous dendrites and accounted for 53% of all submucous neurons. A number of cells resembling enteroendocrine cells in the epithelium of the small and large bowels had intense choline acetyltransferase immunoreactivity. Conclusions: The majority of neurons in human small and large intestines are cholinergic.