Mitotic centromere-associated kinesin is important for anaphase chromosome segregation.
Mitotic centromere-associated kinesin is important for anaphase chromosome segregation.
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DOI：
10.1083/jcb.142.3.787
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发表时间：
1998-08-10
期刊：
影响因子：
--
通讯作者：
Wordeman L
中科院分区：
文献类型：
--
作者：
Maney T;Hunter AW;Wagenbach M;Wordeman L
有丝分裂着丝粒相关驱动蛋白（MCAK）在分裂前期被募集到着丝粒，并保持着丝粒相关直到分裂末期。MCAK是由单个基因编码的同源二聚体，没有相关的亚基。MCAK的无动力版本结合着丝粒但不结合微管，在分裂后期破坏染色体分离。反义诱导的MCAK耗竭导致相同的缺陷。MCAK过表达诱导不依赖于着丝粒的集束和纺锤体微管聚合物的最终损失，这表明着丝粒相关的集束和/或解聚活性是后期所需的。活细胞成像表明，MCAK可能需要协调姐妹着丝粒分离的发病。
Mitotic centromere–associated kinesin (MCAK) is recruited to the centromere at prophase and remains centromere associated until after telophase. MCAK is a homodimer that is encoded by a single gene and has no associated subunits. A motorless version of MCAK that binds centromeres but not microtubules disrupts chromosome segregation during anaphase. Antisense-induced depletion of MCAK results in the same defect. MCAK overexpression induces centromere-independent bundling and eventual loss of spindle microtubule polymer suggesting that centromere-associated bundling and/or depolymerization activity is required for anaphase. Live cell imaging indicates that MCAK may be required to coordinate the onset of sister centromere separation.