Ultracompact 3D microfluidics for time-resolved structural biology
Ultracompact 3D microfluidics for time-resolved structural biology
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DOI：
10.1038/s41467-020-14434-6
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发表时间：
2020-01-31
影响因子：
16.6
通讯作者：
Heymann, Michael
中科院分区：
文献类型：
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作者：
Knoska, Juraj;Adriano, Luigi;Heymann, Michael
需要更稳健的样品递送方法用于连续晶体学。在这里，作者提出了可以打印的超紧凑3D微流体器件的设计和表征，该器件需要较少的样品，具有较低的背景信号，并允许3D混合用于时间分辨实验。为了推进微流体集成，我们提出使用双光子增材制造将2D通道布局折叠成具有纳米精度的紧凑自由形式的3D流体回路。我们通过定制用于X射线自由电子激光器（XFELs）的时间分辨结构生物学的微流体喷嘴和混合器来展示这种技术。我们实现了亚微米射流的速度超过160米秒（-1），这使得使用兆赫XFEL重复率。通过集成一个额外的孔，我们实现了一个低功耗的流聚焦喷嘴，这是通过解决血红蛋白结构进行验证。此外，引入无像差的X射线显微断层成像技术，研究在具有集成到喷嘴中的3D特征的通道中的有效等体积毫秒混合。通过在制造期间局部调整打印分辨率，可以在几分钟内打印这样的设备。该技术有可能通过在微流体工程的所有领域中的3D流动优化来允许超紧凑装置和性能改进。
There is a need for more robust sample delivery methods for serial crystallography. Here the authors present the design and characterization of ultracompact 3D microfluidic devices that can be printed, which require less sample, have a lower background signal and allow 3D mixing for time resolved experiments.To advance microfluidic integration, we present the use of two-photon additive manufacturing to fold 2D channel layouts into compact free-form 3D fluidic circuits with nanometer precision. We demonstrate this technique by tailoring microfluidic nozzles and mixers for time-resolved structural biology at X-ray free-electron lasers (XFELs). We achieve submicron jets with speeds exceeding 160 m s(-1), which allows for the use of megahertz XFEL repetition rates. By integrating an additional orifice, we implement a low consumption flow-focusing nozzle, which is validated by solving a hemoglobin structure. Also, aberration-free in operando X-ray microtomography is introduced to study efficient equivolumetric millisecond mixing in channels with 3D features integrated into the nozzle. Such devices can be printed in minutes by locally adjusting print resolution during fabrication. This technology has the potential to permit ultracompact devices and performance improvements through 3D flow optimization in all fields of microfluidic engineering.